The effects of rootstocks on sugar and acid accumulation and related metabolic enzyme activity during fruit development were studied with Cabernet Sauvignon grafted on four rootstocks and it's self-rooted seedlings as materials.The results showed that glucose and fructose were the main sugars in mature grape fruits,supplemented by sucrose,and tartaric acid and malic acid were the main organic acid components.3309C and SO4 reduced the glucose and fructose content in fruits,but increased the sucrose content significantly.3309C and SO4 significantly increased the contents of tartaric acid and malic acid;1103P increased malic acid content;SO4 and 5BB reduced the citric acid content.From berry enlargement to maturity,the soluble acid invertase(S-AI),neutral invertase(NI),sucrose phosphate synthase(SPS)and sucrose synthetase decomposition direction(SS-I)showed a trend of first increasing and then decreasing,the malate dehydrogenase(NAD-MDH)and phosphoenolpyruvate carboxylase(PEPC)showed a fluctuating downward trend,while the malic enzyme(NADP-ME)and citrate synthase(CS)increased first and then decreased.SO4 decreased the S-AI,SS-c,NADP-ME and CS activity,and increased the SPS,NAD-MDH and PEPC activity.3309C reduced the S-AI,SS-c and NADP-ME activity,and enhanced the SPS,NAD-MDH and PEPC activity.5BB increased S-AI activity during late developmental stage.1103P reduced the SPS and NADP-ME activity,and improved the NAD-MDH and CS activity.The four rootstocks regulate the accumulation and decomposition of sugar and acid in fruit by influencing the enzyme activity of S-AI,SS-c,SPS,NAD-MDH,PEPC and NADP-ME at different stages of fruit development.
NETL
NSTL
万方数据
