首页|引进彩色马铃薯种质资源遗传多样性分析

引进彩色马铃薯种质资源遗传多样性分析

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利用SSR分子标记研究30份引进的彩色马铃薯种质的遗传多样性,明确彩色马铃薯种质资源的亲缘关系.结果表明,17个SSR引物扩增30个种质资源得到139个目标条带,平均每对引物有8.1765个;多态性信息量(PIC)的变化范围为0.4922~0.9989,平均为0.781 7;其中:引物S038和STM0037的PIC最高、条带数最少.UPGMA聚类分析表明,在遗传相似系数SM=0.428处可将参试材料分成3大类群,7(黑美)和29(192306)聚为类群Ⅰ,19[黑美人(宁夏)]和22[圆黑美人(西安)]聚为类群Ⅱ,其余26个材料聚为类群Ⅲ.类群Ⅲ在遗传相似系数SM=0.484处,分为Ⅲ-A群{12[(黑美人(甘肃)]、13(红彩)、24(乌洋芋彩薯)和25(男爵薯)}与Ⅲ-B群(其余22个材料)2个亚群.类群Ⅰ与类群Ⅱ、类群Ⅲ的遗传背景差异较大,可作为彩色马铃薯新品种选育的亲本或中间材料.
Genetic Diversity Analysis of Introduced Color Potato Germplasm Resources
SSR molecular markers were used to study the genetic diversity of 30 introduced colored potato germplasm,and to identify the genetic relationships of colored potato germplasm resources.The results showed that 30 germplasm resources were amplified by 17 SSR primers and 139 target bands were obtained,with an average of 8.176 5 bands per pair of primers.The polymorphism information content(PIC)ranged from 0.492 2 to 0.998 9,with an average of 0.781 7,among which primers S038 and STM0037 had the highest PIC and the lowest number of bands.UPGMA clustering analysis showed that the tested materials could be divided into three groups at the genetic similarity coefficient SM=0.428,7(Heimei)and 29(192306)clustered into groupⅠ,19[Heimeiren(Ningxia)]and 22[Yuan Heimeiren(Xi'an)]clustered into group Ⅱ,and the remaining 26 materials clustered into group Ⅲ.At the genetic similarity coefficient SM=0.484,Group Ⅲ was divided into two subgroups,Ⅲ-A group[12(Heimeiren(Gansu),13(Hongcai),24(Wuyangyu Caishu)and 25(Nanjue Shu)]andⅢ-B group(other 22 materials).The genetic background of Group Ⅰ was different from Group Ⅱ and GroupⅢ,which could be used as parent or intermediate material for breeding new color potato varieties.

Colored potatoGermplasm resourcesGenetic diversity

颉瑞霞、张小川、张国辉、余帮强、吴林科、张丽、郭志乾

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宁夏农林科学院固原分院/国家马铃薯改良中心固原分中心,宁夏 固原 756000

宁夏农林科学院生物技术研究中心,宁夏银川 750002

彩色马铃薯 种质资源 遗传多样性

2024

宁夏农林科技
宁夏农林科学院

宁夏农林科技

影响因子:0.27
ISSN:1002-204X
年,卷(期):2024.65(11)