Objective To investigate the effect of sodium butyrate on the level of inflammatory factors in mice with alcoholic liver disease(ALD)by improving intestinal permeability through enteric-liver circulation.Meth-ods Sixty female C57BL/6J mice were randomly divided into 4 groups:negative control group,ALD model group,sodium butyrate(NaB)-treated control group and NaB-treated ALD model group,15 per group.Mice in the control group were fed with ethanol-free modified Lieber-DeCarli liquid diets,while the model group was fed with ethanol-containing modified Lieber-DeCarli liquid diets.At the same time,the NaB intervention group was fed with liquid feed containing NaB(0.6 g·kg-1),and after 6 weeks of feeding,tissues were collected.HE staining was used to detect intestinal inflammatory cell infiltration and intestinal mucosal integrity.The expres-sion of the intestinal tight junction proteins ZO-1 and Occludin was detected by immunofluorescence.The ex-pression of endotoxin(LPS)in plasma,liver and intestine was detected by limulus amebocyte lysate kit.The ex-pression levels of plasma,liver and intestinal interleukin 6(IL-6),interleukin 1β(IL-1β),and interleukin 17A(IL-17A)were determined by ELISA.The correlation between the tight junction proteins ZO-1 and Occludin with the expression levels of intestinal inflammatory factors(IL-1β,IL-6,IL-17A and LPS)were also analyzed.Results Compared with the negative control group,HE staining results showed in-testinal villus structure of the ALD model group;immunofluorescence results showed decreased expression of tight junction protein(P<0.05);plasma,liver,and intestinal LPS increased(P all<0.05);plasma,liver,and in-testinal inflammatory factor levels increased(P all<0.05).Compared with the ALD model group,intestinal vil-lus structure was increased of the NaB intervention model group;intestinal tight junction protein increased,but plasma,liver and intestinal LPS levels decreased;plasma,liver and intestinal inflammatory factor levels signifi-cantly decreased(Pall<0.05);and correlation analysis results showed negative correlation of intestinal inflam-matory factors(P<0.05).Conclusion Sodium butyrate reduces the levels of inflammatory factors in plasma,liver and intestine of ALD mice by improving intestinal permeability through enteric-liver circulation,thus im-proving the inflammatory response of ALD mice.
维普
万方数据