Mechanistic Study of Sodium Butyrate in Improving Intestinal Permeability and Alcoholic Liver Disease in Mice
Objective To investigate the effect of sodium butyrate on the level of inflammatory factors in mice with alcoholic liver disease(ALD)by improving intestinal permeability through enteric-liver circulation.Meth-ods Sixty female C57BL/6J mice were randomly divided into 4 groups:negative control group,ALD model group,sodium butyrate(NaB)-treated control group and NaB-treated ALD model group,15 per group.Mice in the control group were fed with ethanol-free modified Lieber-DeCarli liquid diets,while the model group was fed with ethanol-containing modified Lieber-DeCarli liquid diets.At the same time,the NaB intervention group was fed with liquid feed containing NaB(0.6 g·kg-1),and after 6 weeks of feeding,tissues were collected.HE staining was used to detect intestinal inflammatory cell infiltration and intestinal mucosal integrity.The expres-sion of the intestinal tight junction proteins ZO-1 and Occludin was detected by immunofluorescence.The ex-pression of endotoxin(LPS)in plasma,liver and intestine was detected by limulus amebocyte lysate kit.The ex-pression levels of plasma,liver and intestinal interleukin 6(IL-6),interleukin 1β(IL-1β),and interleukin 17A(IL-17A)were determined by ELISA.The correlation between the tight junction proteins ZO-1 and Occludin with the expression levels of intestinal inflammatory factors(IL-1β,IL-6,IL-17A and LPS)were also analyzed.Results Compared with the negative control group,HE staining results showed in-testinal villus structure of the ALD model group;immunofluorescence results showed decreased expression of tight junction protein(P<0.05);plasma,liver,and intestinal LPS increased(P all<0.05);plasma,liver,and in-testinal inflammatory factor levels increased(P all<0.05).Compared with the ALD model group,intestinal vil-lus structure was increased of the NaB intervention model group;intestinal tight junction protein increased,but plasma,liver and intestinal LPS levels decreased;plasma,liver and intestinal inflammatory factor levels signifi-cantly decreased(Pall<0.05);and correlation analysis results showed negative correlation of intestinal inflam-matory factors(P<0.05).Conclusion Sodium butyrate reduces the levels of inflammatory factors in plasma,liver and intestine of ALD mice by improving intestinal permeability through enteric-liver circulation,thus im-proving the inflammatory response of ALD mice.
万方数据
维普
