Screening and Analysis of Mitochondria-related Differential Genes in Rat Model of Coal Workers'Pneumoconiosis Based on Transcriptome Sequencing
Objective To screen and analyze mitochondria-related differential genes in the rat model of coal workers'pneumoconiosis by high-throughput transcriptome sequencing.Methods Thirty male SD rats free of specific pathogen(SPF)were selected and divided into the control group,the coal dust group and the silica dust group according to the principle of randomization.The rats were molded by one-time non-exposure endotracheal perfusion and fed for 90 days.The pathological specimens of lung tissue were taken and the pathological changes were observed.The fibrosis was observed by Masson staining.The expression of collagenousⅠ(COL-Ⅰ)in lung tissue was detected by immunohistochemistry to confirm the success of mod-eling.Then,transcriptome sequencing technology was used to analyze the differential expression of genes among the samples and screen out the differentially expressed genes.GO and KEGG bioinformatics analysis were per-formed on the differentially expressed genes,and the differentially expressed genes related to mitochondria in the rats model of coal workers'pneumoconiosis were further screened out and bioinformatics analysis was con-ducted.Results The HE、Masson and ELISA showed that in the coal dust group and silica dust group,COL-Ⅰ increased significantly.There were 35 genes related to mitochondria(including 28 up-regulated and 7 down-regulated)in the control group and the coal dust group.The differential genes of the control group and the silica dust group were screened,and a total of 302 genes related to mitochondria(including 202 up-regulated and 100 down-regulated)were detected.Through GO enrichment analysis,it was found that cellular process and single biological process were more important in biological process.In the molecular function,the active molec-ular sensor and catalytic activity were the main.Among the cell components,cell parts and cells were the main ones.Among the top 20 pathways in which mitochondria-related differential genes in the control group and the dust group were significantly enriched by KEGG,the most genes were enriched in oxidative phosphorylation,thermogenesis,Parkinson's syndrome,prion disease and other pathways.Conclusion By high-throughput transcriptome sequencing,mitochondria-related differential genes in the rat model of coal workers'pneumoco-niosis were screened and analyzed,which provided the basis for further exploration of the relationship between coal workers'pneumoconiosis and mitochondria.
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