首页|膝关节骨性关节炎并内翻畸形股骨髁软骨退行性变观察研究

膝关节骨性关节炎并内翻畸形股骨髁软骨退行性变观察研究

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目的 膝关节骨性关节炎并内翻畸形股骨内侧髁软骨退行性变及超微结构观察.方法 收集 2018 年11 月至 2021 年 10 月行人工全膝关节置换术的 55 例膝关节骨性关节炎并内翻畸形患者的股骨髁,股骨内髁设为观察组,股骨外髁设为对照组.两组分别进行大体观察、HE 染色、番红O-固绿染色及电镜下观察.结果 大体观察:观察组股骨髁关节面不光滑,色泽灰暗,部分软骨剥脱有软骨下骨外露;HE 染色观察:观察组软骨细胞排列不规整,潮线紊乱,软骨下骨板异常增厚,对照组股骨髁软骨退变程度较轻;番红O-固绿染色观察:观察组软骨层着色强度不均匀,番红染色在成簇状软骨细胞周围深染,软骨表面染色不均,软骨基质及细胞数量减少,软骨层与软骨下骨边界不清;扫描电镜下观察:观察组关节面凹凸不平,存在大量凸起、裂隙,对照组表面较为平整,可见软骨表面有散在均匀的软骨颗粒.结论 膝关节骨性关节炎并内翻畸形股骨内髁关节中钙化层和软骨下骨的改变对骨性关节炎发病机制及病情发展起着重要作用.
Morphological and Ultrastructural Observation of Cartilage of Medical Femoral Condyle in Knee Osteoarthritis with Varus Deformtity
Objective To observe degenerative changes and ultrastructure of the medial femoral condylar carti-lage in patients with knee osteoarthritis and varus deformity.Methods The femoral condyles of 55 knee os-teoarthritis patients with varus deformity who underwent artificial total knee arthroplasty from November 2018 to October 2021 were collected.The medial and lateral femoral condyles were divided into observation group and control group.The two groups were subjected to general observation,HE staining,Safranin O-fast green stain-ing,and observation under electron microscope.Results General observation showed that the articular surface of the femoral condyle in the observation group was not smooth,the color was dark,some cartilage was peeled off and the subchondral bone was exposed.HE staining observation showed that the chondrocytes were arranged ir-regularly,the tide line was disordered,and the subchondral bone plate was abnormally thickened.The degree of degeneration of the femoral condylar cartilage in the control group was relatively mild.Safranin O-fast green staining observation showed that the cartilage layer of the observation group has uneven coloring intensity,and the safranin staining was deeply stained around the clustered chondrocytes,and the cartilage surface was stained unevenly.Cartilage matrix and the number of cells reduced,and the boundary between the cartilage layer and the subchondral bone was unclear.Observed under scanning electron microscope showed that t he joint surface of the observation group was uneven,with a large number of bulges and fissures.The surface of the control group was relatively flat,showing that there were even cartilage particles scattered on the cartilage surface.Conclusion The changes in the calcified layer and subchondral bone in the femoral medial condyle joint of knee osteoarthritis and varus deformity play an important role in the pathogenesis and development of os-teoarthritis.

knee jointosteoarthritisvarusmorphologyultrastructure

陈德胜、王胤斌、张晋宁、杨天翔、张博文、郭凤英、李燕

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宁夏回族自治区人民医院骨科,宁夏医科大学第三临床学院,银川 750002

宁夏医科大学,银川 750004

宁夏医科大学基础医学院,银川 750004

膝关节 骨性关节炎 内翻 形态学 超微结构

国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目宁夏回族自治区重点研发计划项目宁夏留学人员创新创业项目宁夏高等学校一流学科建设资助项目宁夏自然科学基金重点项目宁夏医科大学校级课题重点项目

8176040581760395820604082021BEG030492020-75NXYLXK2017A052018AAC02013XZ2018014

2024

10.16050/j.cnki.issn1674-6309.2024.01.009

宁夏医科大学学报
宁夏医科大学

宁夏医科大学学报

CSTPCD
影响因子：0.84
ISSN：1674-6309
年,卷(期)：2024.46(1)
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