miR-145-5P Inhibits the Function of U251 Glioma Cells by Regulating RhoA Protein
Objective To investigate the effect and mechanism of miR-145-5P on the proliferation and invasion of U251 glioma cells.Methods U251 glioma cells were intervened in vitro with miR-145-5P mimic,which were divided into the miR-145-5p overexpression[miR-145-5p(+)]group and the control(NC)group.The effects of miR-145-5p on proliferation,migration and invasion of U251 cells were detected by CCK-8,scratch healing experiment and Transwell experiment.The effects of miR-145-5p on cytoskeleton formation of U251 cells were detected by immunofluorescence experiment,and the expression of RhoA protein in each group were detected by Western blot.Results Compared with the NC group,The results of CCK-8 experiment,scratch healing experiment,and Transwell experiment showed that the OD value,scratch healing rate,and the number of migrating/invading cells of U251 in the miR-145-5p(+)group were significantly reduced(Pall<0.05).The immunofluorescence experiments showed that the cell morphology changed from spindle shaped to circular,with filamentous pseudopodia and patchy pseudopodia wrinkling after the overexpression of miR-145-5p.Western blot experiments showed that the expression of RhoA protein reduced in U251 cells after the overexpression of miR-145-5p(P<0.05).Conclusion miR-145-5p regulates the expression of RhoA protein,thereby affecting the biological functions of glioma cells such as proliferation,migration,and invasion.
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