Objective To explore potential ferroptosis related biomarkers and diagnostic and therapeutic targets in peripheral artery disease(PAD)of lower extremities.Methods Transcriptome data set of PAD of lower limbs were downloaded from gene expression omnibus(GEO),and differential expressed genes(DEGs)were screened using R language,and their intersection with ferroptosis genes was selected.Ferroptosis related differential genes related to lower limb PAD were obtained,and gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment analysis of ferroptosis related differential genes were conducted.The diagnostic value of ferroptosis related differential genes was analyzed by receiver operating characteristic(ROC).Then the protein interaction network was constructed,and the algorithm in Cytoscape was applied to predict key genes.Finally,miRNA targeting key genes were predicted by miRWalk,and the network map was constructed.Results A total of 9 genes related to ferroptosis in PAD lower limbs were screened,and the functional abundance analysis results of GO and KEGG showed that they were related to ferroptosis,immune regulation,apoptosis,diabetes,atherosclerosis,etc.ROC diagnosis suggested that the average area under the curve(AUC)of 9 ferroptosis related differential genes in PAD patients is 0.930.Four key genes were selected based on the algorithm:HMOX1,ALOX5,IL1B and CY BB,while CY BB and HMOX1 were regulated by miR-6734-3p.Conclusion The bioinformatics analysis showed that HMOX1,ALOX5,IL-1B and CY BB have potential roles in inducing ferroptosis in lower limbs PAD,and miR-6734-3p,as a co-targeting miRNA of HMOX1 and CY BB,is expected to become a potential diagnostic biomarker and therapeutic target.
关键词
GEO数据库/下肢外周动脉疾病/铁死亡
Key words
GEO database/peripheral artery disease of lower extremity/ferroptosis
维普
万方数据