摘要
目的 通过生物信息学方法系统性地分析COL6 基因家族各成员在胃癌中的作用.方法 利用TIMER2.0 和GEPIA2 数据库分析COL6 家族基因在胃癌组织中的表达与不同病理分期的关系.通过Kaplan-Meier Plotter数据库分析COL6 基因家族各成员表达水平与胃癌患者总生存期(OS)和无复发生存期(RFS)之间的关系.通过cBioPortal数据库分析胃癌患者中COL6 基因的遗传变异、共表达与邻近基因网络情况.通过STRING数据库和仙桃学术绘制COL6 家族蛋白互作网络图,并进行功能富集分析,分析其作用机制.结果 在胃腺癌中,COL6 基因家族(COL6A1、COL1A2、COL6A3、COL6A4P1、COL6A4P2、COL6A5 和COL6A6)的表达相对于邻近的正常组织上调,且 COL6A1(P=0.000 367)、COL6A2(P=0.000 17)、COL6A3(P<0.001)、COL6A4P1(P=0.864)、COL6A4P2(P=0.355)、COL6A5(P=0.187)和COL6A6(P=0.302)的表达与胃癌患者的病理分期相关.高表达的 COL6A1(P=0.047)、COL6A2(P=0.017)、COL6A3(P=0.02)、COL6A5(P=0.028)和COL6A6(P=0.025)胃癌患者生存率均较低,而 COL6A4P1(P=0.006)、COL6A4P2(P=0.009 1)和 COL6A6(P= 0.002)与患者的转移或复发相关.KEGG 功能富集分析显示差异表达的 COL6A1、COL6A2、COL6A3、COL6A4P1、COL6A4P2、COL6A5、COL6A6 的细胞功能与病灶黏附、细胞外基质受体相互作用信号通路有关.结论 COL6 家族是胃癌预后的潜在生物标志物,且与胃癌的免疫浸润相关.
Abstract
Objective To investigate the role of COL6 gene family members in gastric cancer by bioinformatics methods.Methods In this study,TIMER2.0 and GEPIA2 databases were used to analyze the expression of COL6 family gene in gastric cancer tissues,and their relationship with different pathological stages was analyzed.Kaplan-Meier Plotter database was used to analyze the relationship between the expression levels of COL6 family members and overall survival(OS)and recurrence-free survival(RFS)in gastric cancer patients.Genetic variation,co-expression,and neighboring gene networks of the COL6 gene in gastric cancer patients were analyzed by cBioPortal.STRING database and Xiantao platform was used to draw COL6 family protein interaction network diagram,and functional enrichment analysis was performed to analyze its mechanism.Results In gastric adenocarcinoma,the expression of gene phases of COL6 family(COL6A1,COL1A2,COL6A3,COL6A4P1,COL6A4P2,COL6A5 and COL6A6)was significantly upregulated compared with neighboring normal tissues.A significant correlation was found between the expression of COL6A1(P=0.000 367)、COL6A2(P=0.000 17),COL6A3(P<0.001),COL6A4P1(P=0.864),COL6A4P2(P=0.355),COL6A5(P=0.187)and COL6A6(P=0.302)in the pathological stage of GC patients.GC patients with high COL6A1(P=0.047),COL6A2(P=0.017),COL6A3(P=0.02),COL6A5(P=0.028)and COL6A6(P=0.025)expression were all associated with poor survival,while COL6A4P1(P=0.006),COL6A4P2(P=0.009 1)and COL6A6(P=0.002)were significantly associated with metastasis or recurrence in patients.Subsequently,KEGG functional enrichment analysis showed that its cellular function was associated with focal adhesion,ECM-receptor interaction.Conclusion The COL6 family is a potential biomarker for determining the prognosis of gastric cancer and is associated with immune invasion of gastric cancer.
基金项目
国家自然科学基金(82060663)
国家自然科学基金(82260716)
宁夏回族自治区自然科学基金(2020AAC03178)
宁夏回族自治区自然科学基金(2020A0247)
维普
万方数据