摘要
目的 探讨B细胞淋巴瘤因子 3(Bcl-3)、细胞周期蛋白D1(cyclin D1)、P53 基因编码蛋白和增殖细胞核蛋白(Ki-67)在乳腺导管内增生性病变中的表达及应用价值.方法 采用免疫组化检测乳腺普通型导管增生(UDH)、柱状上皮病变(CCL)、非典型导管增生(ADH)和导管原位癌(DCIS)组织中Bcl-3、cyclin D1、P53 和Ki-67 蛋白的表达.结果 Bcl-3、cyclin D1、P53、Ki-67 蛋白在UDH中均少量表达;在CCL、ADH和DCIS中均明显表达,且随着病变的级别升高,阳性率逐渐增高(P＜0.05);Bcl-3、cyclin D1、P53 和Ki-67 蛋白在CCL、ADH和DCIS中着色强阳性(++～+++)的表达,差异有统计学意义(P＜0.05).结论 Bcl-3、cyclin D1、P53 和Ki-67 蛋白的表达在乳腺导管内增生性病变的病理诊断、鉴别诊断及指导临床治疗方面有较高应用价值,可显著提高乳腺导管内增生性病变的诊断准确率.
Abstract
Objective To investigate the expression and application value of Bcl-3,CyclinD1,P53 and Ki-67 in breast intraductal proliferative lesions.Methods Immunohistochemistry was used to detect the expression of Bcl-3,cyclin D1,P53 and Ki-67 proteins in the tissue of usual ductal hyperplasia(UDH),columnar cell lesion(CCL),atypical ductal hyperplasia(ADH)and ductal carcinoma in situ(DCIS).Results The expression of Bcl-3,cyclin D1,P53 and Ki-67 in UDH were slight.They were significantly expressed in CCL,ADH and DCIS,and the positive rate of the three groups increased gradually(P<0.05).The positive staining(++～+++)of Bcl-3,cyclin D1,P53 and Ki-67 in CCL,ADH and DCIS was statistically significant(P<0.05).Conclusion The expression of Bcl-3,cyclin D1,P53 and Ki-67 has high application value in the pathological diagnosis,differential diagnosis and clinical treatment of intraductal hyperplasia of breast,and can significantly improve the diagnostic accuracy of intraductal hyperplasia of breast.
基金项目
宁夏回族自治区自然科学基金(2022AAC03355)
维普
万方数据