Objective To establish a method for simultaneous determination of orientin,mangiferin,vitexin,verbascoside and hypericin in Jinlianqingre capsules by UPLC-MS/MS.Methods The separations were ob-tained on a Thermo Hypersil GOLD C18(100 mm×2.1 mm,1.9 μm)with a gradient elution using the mixture of acetonitrile and 0.1%formic acid water solution as mobile phase.The flow rate was 0.2 mL·min-1,and the column temperature was set at 35℃.The multiple-reaction monitoring(MRM)scanning was employed for the quantification with switching electrospray ion source polarity in negative mode(ESI-).Results All calibration curves of 5 components were linear(r>0.998)over the tested ranges.The recoveries(n=6)ranged from 98.3%to 101.8%,and the RSD ranged from 1.1%to 1.8%.Conclusion The method is easy to operate,with high accuracy and strong specificity,which can be used for the quality control of Jinlianqingre capsules.
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