Lineage Tracing of Lyz2+Monocytes-macrophages in the Uterus of Polycystic Ovary Syndrome Mice
Objective To establish a polycystic ovary syndrome(PCOS)model on bone marrow-derived macrophage lineage tracing mice,and reveal the distribution of macrophages in the uterus of PCOS through lin-eage tracing.Methods The Cre/LoxP system was used to construct bone marrow-derived macrophage lineage tracer mice,specifically by mating Td-tomatoflax/flax mice with Lyz2Cre tool mice to obtain Lyz2Cre Td-tomatoflax/flax.Red fluorescent protein(Td-tomato)was used to label bone marrow-derived monocytes and macrophages,followed by the construction of a PCOS mouse model using letrozole combined with high-fat feed.The distribution and number of macrophages in the mice uterus were then examined.Results A bone marrow-derived mono-cyte-macrophage lineage tracer mouse(Lyz2Cre Td-tomatoflax/flax)was successfully constructed,and a PCOS mouse model was also successfully established by using this lineage tracer mouse.The HE staining of the uterus of PCOS mice revealed structural abnormalities.Multiple labeling with immunofluorescence detection showed that macrophages in the uterus were distributed in the epimetrium,myometrium,and endometrium,with a significant increase in the number of M2 macrophages in the uterus of the PCOS group(P＜0.05).Conclusion The obese PCOS mouse model was successfully established.The findings indicate a significant increase in bone mar-row-derived M2 macrophages in the uterus of PCOS mice.
万方数据
维普
