Research on Improving the Biocompatibility of Polyaniline Nanomaterials Through Solvent Purification
Objective To investigate the purification effect of organic solvent on the biocompatibility of polyaniline nanomaterials and lay the foundation for the development of novel polymer medical materials.Meth-ods Polyaniline nanofibers synthesized by chemical oxidation were purified with dimethylformamide and dimethyl sulfoxide,respectively and the morphology of original and purified materials was compared.The origi-nal and purified polyanine extracts with 100％,50％,25％,10％,5％and 1％were prepared and the percentage of cell viability was measured by CCK-8 method taking BV2 cells as the object;The original and purified polyaniline suspension with 100 80,64,51 and 41 mg·mL-1 were prepared and the acute oral toxicity and the damage to liver and kidney were also investigated by intragastric administration in mice.Results Polyaniline nanofibers with the diameter of 20 nm were synthesized.After purification,the morphology of the nanofibers did not change.The 100％extract of crude polyaniline had strong cytotoxicity,but the toxicity gradually decreased with the decrease of concentration.The survival rate of cells purified using DMF and DMSO was significantly higher compared to unpurified materials(P all＜0.01).And the cytotoxicity was lower for DMSO-purified PANI materials than DMF-purified ones,but there was no significant difference between two purified materials.The body weight growth rate of mice treated with DMSO-purified material was higher than that of mice treated with crude material(P＜0.05 or P＜0.01)and MTD is larger than 3 000 mg·kg-1 in mice.After dissection,the kidney of mice in each group had no obvious changes,but the hepatocyte injury decreased in mice treated with purified PANI nanofibers.Conclusion Solvent treatment is a simple and feasible purification strategy for polyaniline nanomaterials,which can improve the biocompatibility of the materials and keep the morphology of the materials at the same time.
polyanilinebiocompatibilitycytotoxicityacute toxicity test
万方数据
维普
