The Effects of Different Doses of Selenium on Lipid Deposition in the Aoric Root of Mice
Objective This study aims to investigate the effects of different doses of selenium on lipid deposi-tion in the aoric root of mice.Methods Twenty-seven male C57BL/6J and A poE-/-mice aged 6 to 8 weeks were randomly divided into dietary low selenium group(0.02 μg·g-1),normal selenium group(0.15 μg.g-1)and supplemental selenium group(0.25 μg·g-1),with 9 mice in each group.The C57BL/6J mice were fed with nor-mal diet,and the ApoE-/-mice were fed with high-fat diet(0.15％additional cholesterol,other components were the same as those in the normal diet group containing selenium).After 13 weeks of feeding,the levels of total glutathione peroxidase(GPx),superoxide dismutase(SOD)and malondialdehyde(MDA)were detected by re-duced coenzyme Ⅱ,thiazolium salt and thiobarbituric acid methods respectively.The mRNA and protein levels of selenoproteins glutathione peroxidase 1(GPX1)and glutathione peroxidase 4(GPX4)in the aorta of mice were detected by RT-qPCR and Western blot,and oil-red O staining and HE staining methods were used to detect plaques within the aortic valve and cephalic trunk arteries of mice.Results Compared with the normal seleni-um group,the activity of GPx and SOD in the serum of the low selenium group decreased,while the level of MDA increased(P all＜0.05).The activity of SOD in the serum of the supplemental selenium group increased,while the level of MDA decreased(P all＜0.05).Meanwhile,the levels of GPX1 and GPX4 mRNA and protein in the mice aorta increased(P all＜0.05),compared with the C57BL/6J mice,lipid deposition in the aortic valve and brachiocephalic trunk artery of ApoE-/-mice were reduced(P＜0.05);In ApoE-/-mice,there was significant plaque formation in the brachiocephalic trunk artery;Compared with the normal selenium group,the plaque area in the brachiocephalic trunk artery of mice in the low selenium group increased(P＜0.05),while the plaque area in the brachiocephalic trunk artery of mice in the selenium supplemented group decreased(P＜0.05).Conclusion Appropriate increase of selenium content in the diet enhances the antioxidant capacity in mouse plasma,and prevents the development of lipid deposition in the aoric root in ApoE-/-mice by a mechanism that may be achieved by regulating the expression of GPX1 and GPX4 of mRNA and proteins.
seleniumglutathione peroxidase 1glutathione peroxidase 4lipid deposition in the aoric rootpro-tective effect
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维普
