Objective This study aims to investigate the effects of different doses of selenium on lipid deposi-tion in the aoric root of mice.Methods Twenty-seven male C57BL/6J and A poE-/-mice aged 6 to 8 weeks were randomly divided into dietary low selenium group(0.02 μg·g-1),normal selenium group(0.15 μg.g-1)and supplemental selenium group(0.25 μg·g-1),with 9 mice in each group.The C57BL/6J mice were fed with nor-mal diet,and the ApoE-/-mice were fed with high-fat diet(0.15％additional cholesterol,other components were the same as those in the normal diet group containing selenium).After 13 weeks of feeding,the levels of total glutathione peroxidase(GPx),superoxide dismutase(SOD)and malondialdehyde(MDA)were detected by re-duced coenzyme Ⅱ,thiazolium salt and thiobarbituric acid methods respectively.The mRNA and protein levels of selenoproteins glutathione peroxidase 1(GPX1)and glutathione peroxidase 4(GPX4)in the aorta of mice were detected by RT-qPCR and Western blot,and oil-red O staining and HE staining methods were used to detect plaques within the aortic valve and cephalic trunk arteries of mice.Results Compared with the normal seleni-um group,the activity of GPx and SOD in the serum of the low selenium group decreased,while the level of MDA increased(P all＜0.05).The activity of SOD in the serum of the supplemental selenium group increased,while the level of MDA decreased(P all＜0.05).Meanwhile,the levels of GPX1 and GPX4 mRNA and protein in the mice aorta increased(P all＜0.05),compared with the C57BL/6J mice,lipid deposition in the aortic valve and brachiocephalic trunk artery of ApoE-/-mice were reduced(P＜0.05);In ApoE-/-mice,there was significant plaque formation in the brachiocephalic trunk artery;Compared with the normal selenium group,the plaque area in the brachiocephalic trunk artery of mice in the low selenium group increased(P＜0.05),while the plaque area in the brachiocephalic trunk artery of mice in the selenium supplemented group decreased(P＜0.05).Conclusion Appropriate increase of selenium content in the diet enhances the antioxidant capacity in mouse plasma,and prevents the development of lipid deposition in the aoric root in ApoE-/-mice by a mechanism that may be achieved by regulating the expression of GPX1 and GPX4 of mRNA and proteins.
关键词
硒/谷胱甘肽过氧化物酶1/谷胱甘肽过氧化物酶4/主动脉根部脂质沉积/保护作用
Key words
selenium/glutathione peroxidase 1/glutathione peroxidase 4/lipid deposition in the aoric root/pro-tective effect
维普
万方数据