Effects of Peripheral Blood-derived Exosomes on BV2 Microglia Cells in Mice with Postoperative Delirium
Objective To explore the effects of peripheral blood-derived exosomes on BV2 microglia cells in mice with postoperative delirium(POD).Methods Sixteen C57BL/6J male mice aged 12-14 months were used and randomly divided into control group(Con group)and postoperative delirium(POD)group.Morris water maze and Y maze experiments were used to evaluate whether the POD model was successfully established,and then nanoparticle tracking analysis,transmission electron microscopy,and western blot were used to identify exo-somes in serum,and exosomes were labeled with PKH26 dye.The BV2 cells were randomly divided into three groups:normal group(Nor group),peripheral blood-derived exosomes from normal mice(Con-Exo group),and peripheral blood-derived exosomes from anesthetized/surgical mice(POD-Exo group),and were incubated for a total of 24 hours.Then the activity of the cells in each group was detected by the CCK-8 assay,and the IL-1β,IL-6,and TNF-α relative mRNA and protein expression was detected by RT-qPCR and Western blot.Results Compared with the Con group,the cognitive ability of mice in the POD group was decreased(P＜0.05).The a-bility of BV2 cells to endocytose exosomes labeled with PKH26 dye was observed under confocal microscopy.The activity of BV2 cells in the POD-Exo group was significantly decreased(P＜0.001)compared with that of the Nor and Con-Exo groups and relative mRNA and protein expressions of the pro-inflammatory factors IL-1β,IL-6 and TNF-α increased(P all＜0.001).Conclusion Peripheral blood-derived exosomes in mice with POD can induce inflammatory responses in BV2 cells.
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维普
