Study on the Correlation of Serum 25 Hydroxyvitamin D with M-MDSCs,TNF-α,IL-10 in Patients with Vitamin D Deficiency
Objective To investigate the correlation of 25 hydroxyvitamin D[25(OH1)D]in serum with mono-cytic myeloid-derived suppressor cells(M-MDSCs),tumor necrosis factor-α(TNF-α),and interleukin-10(IL-10)in patients with vitamin D deficiency.Methods A total of 24 patients with vitamin D deficiency(vi-tamin D deficiency group)and 24 healthy donors(normal control group)were recruited to collect basic clinical parameters and peripheral anticoagulant blood,respectivelyfrom the First People's Hospital of Yinchuan City from June to September 2022.After isolation of peripheral plasma and blood cells using centrifugation,flow cytometry was used to detect CD45+white blood cells and CD45+CD15-CD11b+HLA-DR-CD14+M-MDSCs from separated peripheral blood mononuclear cells(PBMCs).ELISA was adopted to separately determine TNF-α and IL-10 levels in serum.Pearson correlation analysis was used to analyze the correlation of 25(OH)D with M-MDSCs and associated inflammatory factors TNF-α and IL-10.Results Compared with normal control group,in vitamin D deficiency group,peripheral white blood cells were significantly increased,M-MDSCs were obviously increased(P all＜0.05),TNF-α level in plasma was notably elevated,whereas IL-10 displayed significant decreased(P all＜0.05).Pearson correlation analysis indicated that 25(OH)D was nega-tively correlated with CD45+white blood cells,M-MDSCs and TNF-α,while positively related to IL-10(P all＜0.05).Conclusion The decrease of 25(OH)D in patients with vitamin D deficiency could promote the occurrence of inflammation which was closely associated with inflammation and M-MDSCs.
vitamin D deficiency25 hydroxyvitamin Dmonocytic myeloid-derived suppressor cellstumor necrosis factor-αinterleukin-10
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维普
