Objective To investigate the effects of gap connexin B3(GJB3)on the proliferation,migration and invasion of lung adenocarcinoma cell H1299.Methods The mRNA and protein levels of GJB3 in human nor-mal lung epithelial cells(BEAS-2B)and lung adenocarcinoma cell lines(H441,A549,H1299)were detected by RT-qPCR and Western blot assay,respectively.The H1299 cell line with the highest expression of GJB3 was selected as the object of follow-up experiment.H1299 cells were transfected with small interfering RNA(siR-NA)and divided into control group(si-NC)and experimental group(si-GJB3).The H1 299 cells viability were detected by CCK-8 after incubation for 0 h,24 h,48 h,72 h and 96 h.The proliferation ability of the cell was detected by plate cloning,the migration ability of the cells were detected by cell scratch assay,and the invasion ability of the cell was detected by Transwell assay,respectively.Results Compared with human normal lung epithelial cells,the expression levels of GJB3 mRNA and protein was higher in lung adenocarcinoma cells,and the expression levels of H1299 cells were the most significant(P＜0.05).After knocking down GJB3 in H1299 cells,the activity migration and invasion ability proliferation ability of the cells was significantly inhibited com-pared with the control group(P＜0.05).After knockdown of GJB3 in H1 299 cells,the of the cells was signifi-cantly inhibited.Knockdown of GJB3 in H1299 cells significantly inhibited the of the cells(P＜0.05).Conclu-sion The expression of GJB3 in lung adenocarcinoma cells is higher than that in normal lung epithelial cells.Knockdown of GJB3 may promote the occurrence and development of lung adenocarcinoma cells.
关键词
间隙连接蛋白B3/肺腺癌/H1299细胞
Key words
gap junction protein beta 3/lung adenocarcinoma/H1299 cells
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