摘要
目的 通过生物信息学方法探索和揭示泛癌中MYD88的功能和分子作用.方法 通过多种生物数据库和工具,从基因表达水平、预后、免疫细胞浸润等多个角度分析MYD88在泛癌中的分子作用.进一步选择5种癌症类型并收集癌组织样本,采用免疫组化技术对上述生物信息学分析结果进行验证.结果 经生物信息学分析及免疫组化分析,在宫颈鳞癌与宫颈腺癌、胶质瘤、卵巢浆液性囊腺癌、多形性胶质母细胞瘤、胃腺癌、乳腺浸润癌组织中的MYD88表达水平高于正常组织(P＜0.05),MYD88表达水平升高与部分癌症患者预后、免疫细胞浸润具有明显相关性(P＜0.05).与MYD88相互作用基因和共表达基因参与了核因子κB(nucle-ar factor kappa-B,NF-κB)信号通路、Toll 样受体(toll-like receptor,TLR)信号通路、NOD 样受体(NOD-like receptor,NLR)信号通路等关键信号通路.另外,这些基因参与了天然免疫应答、炎症反应、信号转导、转录调控、细胞凋亡等多种生物过程.结论 MYD88在多种癌组织中高表达,与患者预后存在相关性,有望成为多种癌症的新型生物标志物.
Abstract
Objective To investigate the function of MYD88 in pan-cancer through bioinformatics methods and the role of MYD88 in the cancer.Methods The role of MYD88 in pan-cancer was investi-gated from various perspectives including gene expression level,prognosis analysis,immune infiltration through various biological databases and tools.Furthermore,cancer tissue samples from five types of cancers were col-lected to confirm the above bioinformatical results by using IHC.Results The expression level of MYD88 in the cervical squamous cell carcinoma,cervical adenocarcinoma,glioma,ovarian serous cystadenocarcinoma,glioblastoma multiforme,gastric adenocarcinoma,and invasive breast cancer was significantly higher than that in normal tissues(P＜0.05),and increased MYD88 expression level was significantly correlated with patient prog-nosis,immune cell infiltration of some patients with cancer(P＜0.05).Genes interacted and co-expressed with MYD88 were involved in various signaling pathways,including nuclear factor kappa-B(NF-κB)signaling pathway,toll-like receptor(TLR)signaling pathway,NOD-like receptor(NLR)signaling pathway and so on.Fur-thermore,the above genes were involved in a series of biological progresses,including innate immune response,inflammatory response,signal transduction,transcriptional regulation,and apoptosis and so on.Conclusion MYD88 was highly expressed in various cancer tissues and exhibits significant differences in TNM system of various cancers.There was a correlation between MYD88 and patient prognosis,and MYD88 was expected to become a new hiomarker for various cancers.
基金项目
国家自然科学基金(81760513)
右江民族医学院研究生创新计划(2023)(YXCXJH2023014)
福建省中青年教师教育科研项目(JAT220084)
维普
万方数据