摘要
目的 使用孟德尔随机化(MR)研究方法验证肠道微生物群(GM)与自闭症谱系障碍(ASD)的因果关系.方法 GM数据来源于MiBioGen联盟进行的全基因组关联研究荟萃分析,包括18 340例受试者;ASD汇总统计数据来自爱丁堡大学开放型全基因组关联研究项目(IEU Open GWAS)发布数据.采用逆方差加权法(IVW)、加权众数和加权中位数估计(WME)进行两样本孟德尔随机化研究;采用Cochran's Q检验、MR-PRESSO和MR Egger截距检验进行敏感性分析,以评估结果的稳定性.结果 Dorea(OR=0.783,95％CI:0.676～0.908)、瘤 胃 球 菌(RuminococcaceaeUCG005)(OR=0.781,95％CI:0.693～0.880)、瘤胃球菌(Ru-minococcaceaeUCG014)(OR=0.863,95％CI:0.761～0.980)和 Victivallis 菌属(OR=0.917,95％CI:0.857～0.981)可能对ASD有保护作用,而巴恩斯氏菌(Barnesiella)(OR=1.182,95％CI:1.047～1.334)、副拟杆菌(Parabac-teroides)(OR=1.213,95％CI:1.017～1.447)和苏黎世杆菌(Turicibacter)菌属(OR=1.129,95％CI:1.007～1.267)可能增加ASD的风险(P均＜0.05).结论 GM和ASD之间具有因果关系,改善GM可能对预防和治疗ASD有一定的临床意义.
Abstract
Objective Investigate possible causal associations between gut microbiota(GM)and autism spectrum disorder(ASD)using two-sample Mendelian randomization(MR).Methods The GM data is sourced from the meta-analysis of genome-wide association studies conducted by the MiBioGen alliance,involving 18 340 subjects,and the ASD meta-analysis is sourced from data released by the Integrated Epidemiology Unit Open Genome-wide Association Study(IEU Open GWAS)project,inverse variance weighted(IVW),weighted mode and weighted median estimator(WME)methods for two-sample MR were used.Cochran's Q test,MR-PRESSO,and MR-Egger intercept test were performed as sensitivity analyses to assess the robustness of the results.Results The results indicate that the Dorea(OR=0.783,95％CI:0.676-0.908),RuminococcaceaeUCG005(OR=0.781,95％CI:0.693-0.880),RuminococcaceaeUCG014(OR=0.863,95％CI:0.761-0.980),and Victivallis(OR=0.917,95％CI:0.857-0.981),may have a protective effect on ASD,while the genera Barnesiella(OR=1.182,95％CI:1.047-1.334),Parabacteroides(OR=1.213,95％CI:1.017-1.447),and Turicibacter(OR=1.129,95％CI:1.007-1.267)may increase the risk of ASD(Pall＜0.05).Conclusion There is a causal relationship between GM and ASD,and improving GM may have clinical benefits in preventing and treating ASD.
维普
万方数据