Objective To investigate the effects of Vitamin C(VC)on apoptosis and invasion of IDH1-mutant glioma cells,evaluate the potential application of VC in antiglioma therapy.Methods Using the human glioma U87 cell line as a foundation,IDH1 mutation was induced by doxycycline(dox+),while the non-induced mutation served as the control group(dox-).Western blot and immunofluorescence were employed to detect IDH1 mutant protein expression,and cell proliferation was assessed using the CCK-8 method.Four groups were established for VC intervention(1 mmol·L-1):VC intervention IDH1 mutation group(dox+VC+),IDH1 mutation group without VC intervention(dox+VC-),non-IDH1 mutation group with VC intervention(dox-VC+),and non-IDH1 mutation group without VC intervention(dox-VC-).Cell invasion ability was evaluated using the Transwell assay,while Western blot was utilized to detect apoptosis,invasion-related proteins,and stress-related factors expression.Cell proliferation was also assessed using the CCK-8 method.Results Cell proliferation in the dox+group with IDH1 mutation was significantly reduced(P＜0.05).After VC intervention,the expression of apoptotic proteins Bax,Bad,Cleaved-caspase-3,Cleaved-PARP in both IDH1-mutant and non-mutant glioma cells was upregulated(P all＜0.05),while the anti-apoptotic protein Bcl-2 and invasion-related protein family members MMP-7,MMP-9 expression decreased(P all＜0.01).The expression of the stress-protective protein p-HSP27 was upregulated(P＜0.01),and the number of invasion cells significantly decreased(P＜0.05).Conclusion VC promotes apoptosis and inhibits invasion of IDH1-mutant glioma cells.
维普
万方数据