Protective Effect and Mechanism of Buqing Granule on Liver Injury in Type 2 Diabetic Rats Based on Keap1-Nrf2 Signaling Pathway
Objective To investigate the protective effect of Buqing granule on liver injury in type 2 diabetes mellitus(T2DM)rats and its mechanism based on Keap1-Nrf2 signaling pathway.Methods T2DM was induced by high-fat and high-sugar diet combined with low-dose streptozotocin,and then the T2DM model with liver injury was established by continuous high-fat and high-sugar diet.The control group,model group,high-dose Buqing granule group,and middle-dose Buqing granule group were set up in the experiments.After 8 weeks of administration,the blood glucose,liver function,and oxidative stress related indexes of rats in each group were detected.HE staining was used to observe the pathological morphology of liver tissue.Western blot and RT-qPCR were used to detect the expression level of Keap1-Nrf2 pathway-related proteins and mRNA expression in rat liver.Results Compared with the control group,the rats in the model group had significant weight loss,blood sugar increase,and liver function damage(P all＜0.05).The weight,blood sugar and liver function in the Buqing granule group were improved compared with the model group(P all＜0.05).The oxidative stress-related indexes in the Buqing granule group were improved compared with the model group(P＜0.05).The expression of Keap1 and Cytoplasm Nrf2 protein in each dose group of Buqing granule was lower than that in the model group,while the expressionlevels of Nuclear Nrf2,NQO1 and HO-1 were higher than those in the model group(P all＜0.05).The expression of Nrf2,NQO1 and HO-1 mRNA in each group of Buqing granule was up-regulated compared with the model group(P all＜0.05).Conclusion Buqing granule can reduce liver injury in T2DM rats,improve the general condition and pathological changes of liver tissue in rats,and its mechanism may be related to the regulation of Keap1-Nrf2 signaling pathway to combat antioxidant damage.
type 2 diabetesliver injuryBuqing granuleoxidative damageKeap1-Nrf2 pathway
万方数据
维普
