Proteomic Analysis of Differentially Expressed Proteins after Intravenous Lidocaine
Objective To analyze the differential protein expression in serum before and after intravenous injection of lidocaine(IVL)using proteomics techniques.Methods Five healthy volunteers were enrolled in the study.5 mL of venous blood samples were taken from five volunteers before intravenous lidocaine,and then lidocaine was given intravenously at a dose of 1.5 mg·kg-1 for 5 minutes,followed by continuous pump infusion at 3 mg·(kg·h)-1 for 30 minutes.5 mL of venous blood was collected 1 h after the end of the pump.Using tandem mass tag labeling and high-performance liquid chromatography-tandem mass spectrometry,differentially expressed proteins were identified after intravenous lidocaine.The biological information of proteins was analyzed using the Kyoto encyclopedia of genes and genomes(KEGG)and gene ontology(GO)databases.Results A total of 15 differentially expressed proteins were identified before and after intravenous lidocaine.Among them,6 proteins were up-regulated and 9 proteins were down-regulated.GO analysis revealed that most differentially expressed proteins were involved in cellular processes,and subcellular localization analysis showed that most proteins were located in the extracellular region and cytoplasm.Enrichment analysis revealed that most proteins were involved in inflammation regulation,regulation of B cell activation,protein processing.KEGG pathway enrichment of the up-regulated proteins analysis revealed the p53 signaling pathway.By studying the differentiallyexpressed proteins of the up-down-regulated group,it was found that Etoposide-induced protein 2.4(EI24)of the up-regulated group was involved in the p53 pathway and regulated the calcium ion concentration.Conclusion IVL may inhibit the development of tumor cells by regulating etoposide-induced protein 2.4(EI24)and Suprabasin.EI24 may participate in the sedative effect of IVL by regulating intracellular calcium ion concentration.
differentially expressed proteingene ontologyenrichment analysistandem mass taglidocaine
万方数据
维普
