Exploring the Impact of STARD8 Expression on the Biological Function of Lung Cancer A549 Cells Based on Bioinformatics Analysis
Objective To investigate the effects of STARD8 on the biological functions of proliferation,migration,invasion,and apoptosis in lung cancer A549 cells.Methods Two publicly available gene-on-a-chip datasets(GSE18842 and GSE68571)were acquired from the National Center for Biotechnology Information(NCBI).The limma R package was utilized to identify differentially expressed genes(DEGs).A combination of the minimum absolute contraction and selection operator(LASSO)regression method with the SVM-RFE algorithm was employed to screen candidate genes,receiver operating characteristic(ROC)curves was constructed,and immunoinfiltration analysis was performed by using the CIBERSORT tool.The mRNA and protein levels of STARD8 in both the control group and lung cancer group were assessed through RT-qPCR and Western blot.The impact of STARD8 on proliferation,invasion,migration,and apoptosis of A549 cells was evaluated via cell plate cloning,Transwell assay,scratch assay,and flow cytometry.Results A total of 285 up-regulated genes and 377 down-regulated genes were screened,and a diagnostic marker gene STARD8 was obtained by intersection.Immunoassay showed immunosuppression in CD4 T cells and NK cells.The mRNA and protein expression of STARD8 in the lung cancer group was down-regulated(P all＜0.05).When STARD8 was overexpressed,the proliferation,invasion and migration ability of A549 cells of lung cancer decreased,while the apoptosis ability increased(P all＜0.05).Conclusion STARD8 can inhibit the proliferation,invasion and migration of lung cancer A549 cells,and promote apoptosis.
lung cancer A549 cellsbioinformaticsGTP enzyme activating factorproliferationinvasionmigrationapoptosis
万方数据
维普
