Study on the Effects of Free Drinking Water Exposure to Acetamiprid on the Testes of Male Mice
Objective To establish the testicular injury model of male mice induced by acetamiprid and explore the damage of male reproductive system induced by acetamiprid.Methods 24 SPF grade C57BL6N male mice were selected and randomly divided into 4 groups,with 6 mice in each group.They were respectively set as the control group(pure water),the low-dose group(0.64 mg·kg-1),the medium-dose group(9.1 mg·kg-1),and the high-dose group(18.2 mg·kg-1).Male mice were exposed to poison in drinking water to create models,and the exposure continued for 28 days.The body weights of mice collected the next day and the changes in body weight and testicular organ coefficients of the mice were observed in each group.The changes in sperm quality of the mice were observed through sperm smears.The pathological changes in testicular tissue were checked through HE staining.Results There were no statistical difference in the body weight of mice in each group before and after acetamiprid exposure(P all＞0.05).At the same time,compared with the control group,there was no statistically significant difference in testicular organ coefficients among the low,medium and high dose groups(Pall＞0.05).Through sperm smears of the mice,it was observed that as the dose of poisonincreased,the number of sperm gradually decreased and the sperm morphology changed.HE staining results showed that compared with the control group,as the dose of poison increased in the low,medium and high dose groups,the cells in the seminiferous tubules were loosely arranged,the density of sperm in the lumen decreased,and some cells fell off.Conclusion Acetamiprid poisoning can cause damage to spermatogenic cells in the testicles of male mice,and lead to a decrease in the sperm count and changes in the sperm morphology.
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