The Cancer-promoting Function of CIRBP on Human Ovarian Cancer Cell SKOV3
Objective To explore the cancer-promoting effect of cold inducible RNA binding protein(CIRBP)on human ovarian cancer cell SKOV3.Methods CIRBP expression in ovarian cancer was analyzed using bioinformatics.The SKOV3 cell line stably transfected by lentivirus with CIRBP gene silencing and overexpression was established.CCK-8 was used to detect cell proliferation,flow cytometry was applied to detect cell apoptosis and cell cycle,scratch assay was used to detect cell migration,and cellular immunofluorescence was used to detect the nuclear translocation level of β-catenin,CIRBP mRNA expression was detected by RT-qPCR,CIRBP,β-catenin and p-β-catenin protein expression levels were detected by Western blot.Results The expression of CIRBP was significantly downregulated in ovarian cancer(P＜0.05).CIRBP silenced and overexpressed cell models were satisfactorily constructed.CIRBP silencing resulted in a significant decrease in cell proliferation(P＜0.05),a significant increase in apoptosis(P＜0.05),a sizable decrease in migration ability(P＜0.05),and the proportion of cells in G0/G1 phase increased,the nuclear translocation level of β-catenin decreased(P＜0.05),the protein expression of β-catenin was down-regulated(P＜0.05),while the protein expression of p-β-catenin was significantly up-regulated(P＜0.05),and CIRBP over expression was reversed.Conclusion CIRBP promotes the expression and nuclear translocation of β-catenin,thereby affecting the progression of ovarian cancer.
ovarian cancerSKOV3 cellscold induced RNA binding proteinfunction and mechanism
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