Expressions of TIPE2 in The Process of Periodontitis
Objective This study aims to explore the expression of TIPE2 in periodontal tissues with different degrees of eriodontitis and development mechanism of periodontitis.Methods Firstly,12 patients with periodontitis who underwent periodontal surgery were selected,and patients with a history of systemic diseases were excluded.According to the severity of periodontitis,the volunteers were divided into severe group(n=6)and mild-moderate group(n=6).Then,immunohistochemical staining was performed on the gingival tissues excised during surgery to observe the expression of TIPE2 in gingival tissues with different degrees of periodontitis.Next,human periodontal ligament cells(PDLSC)were cultured in vitro and stimulated with 1 μg·mL-1 Pg-LPS to simulate the inflammatory environment.Immunohistochemical staining and RT-qPCR were performed to detect the gene expression level of TIPE2 in PDLSC under inflammatory conditions.Results The number of TIPE2-positive inflammatory cells infiltrating in severe group gingival tissues was significantly higher than that in mild-moderate group(P＜0.05);Immunohistochemical staining indicated positive expression of TIPE2 in PDLSC cultured in vitro;After stimulating PDLSC with Pg-LPS in the inflammatory microenvironment for 24 h,48 h,72 h,RT-qPCR showed that the gene expression level of TIPE2 in cells decreased compared with that in control group PDLSC.Conclusion TIPE2 is involved in the pathogenesis of periodontitis in humans and is closely related to the severity of inflammation.The gene expression level of TIPE2 in PDLSC decreases in the inflammatory microenvironment.The mechanism of action of TIPE2 in the progression of periodontitis needs further exploration.
periodontitishuman periodontal ligament cellstumor necrosis factor α-induced protein 8-like protein 2lipopolysaccharide
万方数据
维普
