The Expression and Significance of HSP70,DLAT and FDX1 in Myocardial Ischemia-reperfusion Injury
Objective To investigate the expression of copper death-related genes HSP70,DLAT and FDX1 in myocardial cells of rats with myocardial ischemia/reperfusion(I/R).Methods The rats'model of myocardial I/R was established by ligating the left anterior descending branch of the coronary artery.Forty SPF male rats were randomly divided into four groups:the first week model group(Model group),the first week sham operation group(Sham group),the fourth week model group(Model'group)and the fourth week sham operation group(Sham'group).Cardiac magnetic resonance imaging(CMR)was performed at the first week and the fourth week after successful modeling.HE and Masson staining were used to observe the pathological changes of myocardial tissue.Western blot and RT-qPCR were used to detect the protein and mRNA expressions of HSP70,DLAT and FDX1 in myocardial tissue.Results In the first week,compared with the Sham group,the Model group had serious pathological injury of myocardial tissue,decreased cardiac function and significant increases in the expression levels of HSP70,DLAT,and FDX1 protein and mRNA in myocardial tissue(P＜0.05).At the fourth week,compared with the Sham'group,the Model'group had serious pathological damage of myocardial tissue,further reduced cardiac function and significantly increased expression levels of HSP70,DLAT,and FDX1 protein and mRNA in myocardial tissue(P＜0.05).Conclusion Cuproptosis may play a role in the pathogenesis of myocardial I/R injury by up-regulating HSP70,DLAT and FDX1 proteins in cardiomyocytes.
万方数据
维普
