Expression and Clinical Significance of Silenct Information Regulator 1 and Hypoxia Inducible Factor-1α in Cutaneous Malignant Melanoma
Objective To investigate the expression of SIRT1 and HIF-1 α in cutaneous malignant melanoma(CMM)and analyze their relationship with clinical factors.Methods A total of 30 surgical specimens of CMM and melanocytic nevus confirmed by histopathology and immuno-histochemistry were collected from the Department of Dermatology of General Hospital of Ningxia Medical University from February 2022 to February 2024.The expressions of SIRT1 and HIF-1 α were detected by RT-qPCR and immunohistochemical method in CMM and melanocytic nevus tissue samples.Chi-square test was used to analyze and evaluate the correlation between the expression of SIRT1 and HIF-1 α and the gender,age,tumor site,tumor lesion diameter and pathological grade of patients.Results RT-qPCR results showed that the relative expression level of SIRT1 mRNA in melanocytic nevus was lower than that of CMM(P＜0.01).The relative expression level of HIF-lα mRNA in melanocytic nevus was lower than that of CMM(P＜0.05).Immunohistochemical experiment results showed that the expression of SIRT1 in CMM was significant high than that in melanocytic nevus(t=10.14,P＜0.001),and the expression of HIF-1α increased in CMM,which was significant high than that in melanocytic nevus(t=21.99,P＜0.001).The expression intensity of SIRT1 was correlated with tumor Clark grade and lymph node metastasis(P all＜0.05).The expression intensity of HIF-1 α was correlated with tumor grade,site and lymph node metastasis(P all＜0.05).Conclusion The expression of SIRT1 and HIF-1 α in CMM tissues were increased,and they were related to Clark grade,lymph node metastasis,and tumour site,which could be used as biomarks to predict tumor invasion,metastasis and prognosis.
cutaneous malignant melanomamelanocytic nevussilent information regulator 1hypoxia inducible factor-1α
万方数据
维普
