摘要
目的 探讨不同玻璃化冷冻时间对冻胚复苏及移植妊娠结局的影响.方法 回顾性分析进行冻胚移植(FET)的550例患者的临床资料,根据胚胎不同玻璃化冷冻时间分为A、B、C、D、E共5组,A组239例,玻璃化冷冻时间≤3个月;B组138例,3个月＜玻璃化冷冻时间≤6个月;C组74例,6个月＜玻璃化冷冻时间≤1年;D组75例,1年＜玻璃化冷冻时间≤5年;E组24例,玻璃化冷冻时间＞5年.比较不同冷冻时间胚胎移植的一般临床资料、胚胎复苏结局及临床妊娠结局.结果 相较于A、B、C组,D组胚胎存活率、胚胎完好率显著降低(P＜0.05);相较于A、B组,E组的胚胎存活率、胚胎完好率及解冻后/冷冻前优质胚胎率均显著降低(P＜0.05);E组的胚胎完好率较C组显著降低(P＜0.05);E组的解冻后/冷冻前优质胚胎率显著低于D组(P＜0.05).各组间移植优质胚胎后的胚胎种植率、临床妊娠率、活产率、流产率及早产率差异无统计学意义.移植优质卵裂期胚胎后,E组早产率较B组显著增高(P＜0.05).移植优质囊胚后,C组早产率较A组显著增高(P＜0.05).结论 在冻胚移植周期中,较长的玻璃化冷冻时间对胚胎的复苏结局存在不利影响,但在移植至少一枚优质冷冻胚胎后,不同冷冻时间对胚胎移植临床妊娠结局并无影响.
Abstract
Objective To explore the effects of different vitrification freezing times on frozen embryo recovery and pregnancy outcomes.Method A retrospective analysis was conducted on the clinical data of 550 patients who met the inclusion criteria and under-went frozen embryo transfer.According to different vitrification freezing times of embryos,they were divided into 5 groups:A,B,C,D and E.Group A was 239 cases,with vitrification freezing time ≤3 months;Group B was 138 cases,with 3 months＜vitrification freezing time≤ 6 months;Group C was 74 cases,with 6 months＜vitrification freezing time ≤ 1 year;Group D was 75cases,with 1 year＜vitrification freezing time≤ 5 years;Group E was 24 cases,with vitrification freezing time＞5 years.The general clinical data,embryo recovery out-comes and clinical pregnancy outcomes of embryo transfer with different vitrification freezing times were compared.Results Compared with groups A,B,and C,the embryo survival rate and embryo integrity rate in group D were significantly reduced(P＜0.05).Compared with group A and B,the embryo survival rate,embryo integrity rate,and high-quality embryo rate after thawing/before freezing in group E were significantly reduced(P＜0.05).The embryo integrity rate of group E was significantly lower than that of group C(P＜0.05).The rate of high-quality embryos after thawing/before freezing in group E was significantly lower than that in group D(P＜0.05).There were no statistically significant differences in embryo implantation rate,clinical pregnancy rate,live birth rate,miscarriage rate,and premature birth rate among the groups after transferring high-quality embryos.After transferring high-quality cleavage stage embryos,the premature birth rate in group E was significantly higher than that in group B(P＜0.05).After transplantation of high-quality blas-tocysts,the premature birth rate in group C was significantly higher than that in group A(P＜0.05).Conclusion In the frozen embryo transfer cycle,a longer vitrification freezing time has a negative impact on the recovery outcome of embryos,but after transferring at least one high-quality frozen embryo,different vitrification freezing times have no effect on the clinical pregnancy outcome of embryo transfer.
维普
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