Effects of Tongxinluo capsule on cardiac function and myocardial remodeling in diabetes rats
Objective To study the effects of Tongxinluo capsule on cardiac function and myocardial remodeling in diabetes rats.Methods Eighty three male SD rats were randomly divided into high,middle and low dose of Tongxinluo groups,valsartan group and diabetes group after being modeled with 2％STZ(30 mg/kg).The treatment group was gavaged for 10 weeks and the general condi-tion of the rats was observed;Body weight and blood sugar were monitored;Cardiac function was also measured after anesthesia in rats,and cardiac mass index was measured after heart extraction.The expression of TGFβ1,Caspase-3 and bcl-2 proteins were measured by using ELISA.Results General situation:The high,medium and low dose groups of Tongxinluo capsule and valsartan groups were more active in mental state,shiny in hair color,and the symptoms of overeating,drinking and urination are alleviated,the stool was dry,and the body mass was increased compared with the diabetes group.Weight comparison:After modeling,Tongxinluo high,medium and low dose groups,valsartan group and diabetes group significantly decreased compared with the blank control group.At 4-10 weeks of intragastric administration,the difference between each group and the control group was statistically significant(P＜0.05).Blood glucose compari-son:at 1-10 weeks after intragastric administration,the indexes of Tongxinluo high,medium and low dose groups,valsartan group and di-abetes group were significantly higher than those of the control group(P＜0.05),and there was no statistical significance between diabe-tes group and other experimental groups(P＞0.05).Comparison of cardiac function indexes:comparison of ventricular septal thickness,posterior wall thickness,ventricular end diastolic diameter,left ventricular end systolic diameter,left ventricular end diastolic volume,and left ventricular end systolic volume,all indexes of valsartan and Tongxinluo high-dose groups were significantly lower than those of dia-betes group(P＜0.05).Compared with diabetes group,ejection fraction and short axis shortening rate were significantly higher in each group(P＜0.05);The high-dose group of Tongxinluo showed a significant increase in ejection fraction compared to the valsartan group(P＜0.05).Comparison of cardiac mass index:the whole heart mass index and left heart mass index of valsartan group and Tongxinluo high-dose group were significantly lower than those of diabetes group(P＜0.05).Comparison of protein expression of TGF-β1 and Caspase-3:The ratio of valsartan group and Tongxinluo high and middle dose groups was significantly lower than that of diabetes group(P＜0.05);There was no statistically significant difference(P＞0.05)between the valsartan group and the high-dose Tongxinluo group.Bcl-2 index:Valsartan and Tongxinluo high and middle dose groups were significantly higher than diabetes group(P＜0.05);The high-dose group of Tongxinluo was significantly higher than the valsartan group(P＜0.05).Conclusion Tongxinluo capsule can significantly improve the heart function and inhibit myocardial remodeling in diabetes rats,with considerable effect.
万方数据
维普
