The value of second-generation gene sequencing in the determination of early causative agents in severe pneumonia
Objective To investigate the value of second-generation gene sequencing technology(mNGS)in the determina-tion of early causative organisms in severe pneumonia.Methods Selected 32 patients,General clinical data,laboratory tests,pulmonary imaging data,28-day survival rate and ICU treatment time.Results The test results return in group A took(23.65±3.20)hours and in group B it took(117.46±16.32)hours.Group A took significantly less time than group B(P＜0.05);Group A had higher sensitivi-ty than group B(P＜0.05),while specificity was lower than group B(P＜0.05).The positive predictive value in group A was 88.97％and the negative predictive value was 58.97％,while the positive predictive value in group B was 98.72％and the negative predictive value was 76.09％;26 patients adjusted their antibiotic treatment strategy based on empirical anti-infection treatment according to the early mNGS results,combined with the patient's temperature and inflammatory index,and 21 of them improved after changing the medica-tion regimen,5 patients died.Of the 32 patients registered,7 died,and the 28-day mortality rate was 21.9％.Conclusion mNGS is clinically valuable for the diagnosis of early causative organisms in severe pneumonia and it can improve the diagnostic accuracy and ra-pidity of this disease.
万方数据
维普
