Nurr1 attenuates microglia inflammation by inhibiting NF-κB
Objective To explore whether Nurr1 inhibits NF-κB expression and nuclear translocation in BV2 microglia inflam-matory response induced by LPS to play an anti-inflammatory role.Methods Mouse BV2 microglia cells were cultured and divided in-to Ctrl group,LPS group,C-DIM12 group(Nurr1 specific agonist),siRNA interference group and LPS+C-DIM12 group.CCK-8 was used to detect the optimal concentration of LPS and C-DIM12,and the expression changes of NF-κB and Nurr1 were measured by Western blot,and the inflammatory factors IL-6,IL-1β and TNF-α levels were detected by ELISA.After the expression activity of Nurr1 was disrupted by siRNA or activated by C-DIM12,the expression levels of NF-κB and p-IκB in BV2 cells were investigated.Results After the BV2 cells were stimulated by LPS,the expression of NF-κB increased and the release of inflammatory factors IL-1,IL-6,TNF-α also increased(P<0.05).After Nurr1 was activated by C-DIM12,the expression of NF-κB decreased signif-icantly.However,after small interfering RNA(siNurr1)silenced the expression of Nurr1,the expression of NF-κB increased.In LPS-activated microglia,the level of phosphorylated protein I-κB,which was NF-κB dimer molecule binding molecule,was significantly higher than that in the control group.After Nurr1 was activated by C-DIM12,pI-κB level decreased significantly.Conclusion Nurr1 can inhibit the expression of NF-κB,reverse NF-κB nuclear translocation and protect the CNS from inflammation.Nurr1 may be a new and potential target for the treatment of inflammation related CNS diseases.
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