Study on the mechanism of synovial repair induced by TLR4/NF-kB signaling pathway induced by warm acupuncture on rabbit knee osteoarthritis model
Objective To investigate the effect of warm acupuncture on KO A by observing the changes of TLR4 and NF-κB expression in articular cartilage synovial tissue of rabbit knee osteoarthritis(KOA)model.Methods 40 rabbits were randomly divided into blank group,model group,warm acupuncture treatment group,and diclofenac sodium drug group(n=10).KOA model was made by right hind limb plaster cast fixation,and intervention treatment was started on the third day.The blank group did not receive any treatment and was fed routinely;the model group did not receive any treatment and was fixed once a day for a fixed period of 15 minutes.The warm acupuncture-moxibustion treatment group was treated with warm acupuncture and moxibustion at the affected side of Heding,Housanli,Neixianyan,and Yanglingquan,once a day for 15 minutes each time.In the diclofenac sodium group,the drug powder was dissolved in pu-rified water and administered by gavage at a concentration of 15 mg/kg.All groups took six days as a course of treatment,a total of two courses of treatment,and the materials were collected after the treatment.HE staining was used to observe the pathological changes of knee articular cartilage in each group,and Mankins score was performed;ELISA method was used to detect the secretion levels of NF-κB and TLR4 in the synovial tissue of each group.Results Compared with blank group,Mankin's score,NF-κB and TLR4 in all groups were increased(P＜0.05).Compared with model group,Mankin's score,NF-κB and TLR4 contents in warm acupuncture and moxibustion group and diclofenac sodium group were decreased(P＜0.05).Conclusion Warm acupuncture can improve the degenera-tion of rabbit knee articular cartilage and reduce the inflammatory response.The mechanism may be related to improving the shape of rab-bit knee articular cartilage and reducing the expression levels of NF-κB and TLR4 in synovial tissue.
Warming acupunctureKOA of RabbitSynovial repairTLR4/NF-κB signaling pathway
NETL
NSTL
万方数据
