Study on the improvement of high glucose-induced damage to retinal pigment epithelial cells by ferulic acid
Objective To observe the effect of ferulic acid(FA)on the injury of retinal pigment epithelial cells(ARPE-19)induced by high glucose(HG),and to explore the mechanism of FA on diabetes retinopathy.Methods The model of diabetic retinopa-thy in vitro was constructed by culturing ARPE-19 with HG,and FA was dissolved in dimethyl sulfoxide(DMSO).They were divided into Control group(5 mmol/L glucose),DMSO group(0.1％DMSO+5 mmol/L glucose),HG group(30 mmol/L glucose)and HG+FA group(30 mmol/L glucose+50 μmol/L).The proliferative activity of the cells was detected by CCK-8 assay.ROS levels of ARPE-19 cells were detected by reactive oxygen species(ROS)detection kit.The expression levels of TLR4,MyD88 and NF-κB were detected by immunofluorescence and Western blotting.Results Compared with the control group,HG decreased the proliferation activity of ARPE-19 cells,while FA ameliorated the effect of HG on the proliferation of cells(P＜0.05).Compared with the control group,the ROS fluorescence intensity of ARPE-19 cells induced by HG increased,and the FA significantly decreased the ROS fluores-cence intensity of ARPE-19 cells induced by HG(P＜0.05).Immunofluorescence and Western blot experiments showed that the ex-pression of TLR4,MyD88 and NF-κB in ARPE-19 cells increased in HG group,while they significantly decreased in FA group(P＜0.05).Conclusion FA can improve the damage of retinal pigment epithelial cells induced by HG by decreasing ROS production and down-regulating the expression of TLR4,MyD88 and NF-κB.
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