Study on the mechanism of inulin remodeling gut microbiota in db/db mice with type 2 diabetes mellitus
Objective To investigate the effect of inulin on T2DM via refactoring gut microbiota in mice.Methods 20 db/db leptin receptor mutant mice were used as the T2DM,According to previous research methods,model mice were randomly allocated into inulin treated group(INU/DM group,n=10)andnon-treatedgroup(DMgroup,n=10)and10 C57BL/6J mice were used as the neg-ative control group.5%(wt/wt)inulin was orally administered to the treated groups for six weeks,and an equal volume of saline was orally administered to the untreated groups.Body weight,blood glucose,glycated hemoglobin,blood lipids and LPS levels were detected in each group.16S rRNA sequencing was used to analyze the changes of gut microbiota.Results Blood glucose,glycated hemoglobin,blood lipids and LPS levels were significantly decreased in INU/DM group compared to DM group(P<0.05).Declined LPS level was ob-served in INU/DM group compared with that in DM group(P<0.05).The supplementation of inulin significantly altered the beta diver-sity and relative abundance of the gut microbiota by increasing the relative abundance of anti-diabetic cyanobacteria and SCFAs-pro-ducing bacteroidaceae and genus levels(P<0.05).Concurrently,inulin reduced the relative abundances of pathogenic bacteria inclu-ding deferribacteres,tenericutes,ruminococcaceae,and ruminclostridium_6(P<0.05).Conclusion Inulin could ameliorates T2DM by reshaping the gut microbiota and inhibiting LPS translocation.
Type 2 Diabetes MellitusInflammationInulinGut microbiota
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