Effects of high fat diet induced obesity on the structure and function of hypothalamus mitochondria
Objective To study the effect of obesity induced by high fat diet on the structure and function of hypothalamus mi-tochondria in mice.Methods Twelve male C57BL/6J mice,aged six weeks,were randomly assigned to two groups:a group fed with a high-fat diet(HFD)and a group fed with a normal diet(NC).After 12 weeks of being on their respective diets,various measurements such as body weight,blood lipid levels,Hematoxylin-Eosin staining(HE),and Oil-red O staining of the liver were conducted in order to assess the successful establishment of an obesity model.The presence of ROS was identified using a reactive oxygen species(ROS)detection kit,while the levels of MDA and SOD were measured using malondialdehyde(MDA)and superoxide dismutase(SOD)kits respectively.The transmission electron microscope was utilized to observe the ultrastructure of mitochondria in the hypothalamus,and the mitochondrial membrane potential(MMP)was assessed using an MMP(JC-1)detection kit.Additionally,luciferase enzyme was em-ployed to measure ATP content.Results Compared with NC group,body weight,serum total cholesterol(TC),high density lipoprotein(HDL),low density lipoprotein(LDL)contents and liver lipid deposition of HFD group were significantly increased(P<0.05),hypo-thalamic SOD activity of HFD group was decreased(P<0.05),while MDA content was increased(P<0.05).HFD group mice hypo-thalamus mitochondrial edema,mitochondrial crest fracture,mitochondrial membrane potential and ATP content decreased(P<0.05).Conclusion Obesity induced by high-fat diet can cause structural disorder and dysfunction of hypothalamic mitochondria in mice.
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