高脂饮食诱导的肥胖对下丘脑线粒体结构及功能的影响
Effects of high fat diet induced obesity on the structure and function of hypothalamus mitochondria
邢欣然 1刘祺 2杜勇 2罗佳奇 1高燕 1苏泽礼 1王凯2
作者信息
- 1. 宁夏医科大学总医院小儿外科,宁夏 银川 750004
- 2. 宁夏医科大学临床医学院,宁夏 银川 750004
- 折叠
摘要
目的 研究高脂饮食诱导的肥胖对小鼠下丘脑线粒体结构及功能的影响.方法 选取12 只6 周龄的C57BL/6J雄性小鼠,按照随机数字表法将其分为高脂饮食组(HFD)、正常饮食组(NC),分别给予高脂饮食及正常饮食12 周后,通过测量体重、血脂、肝脏苏木素-伊红(HE)染色及油红O染色来判断肥胖模型是否构建成功;活性氧(ROS)检测试剂盒检测ROS含量,采用丙二醛(MDA)、超氧化物歧化酶(SOD)试剂盒检测MDA含量及SOD酶活性,透射电镜观察下丘脑线粒体的超微结构,采用线粒体膜电位(MMP)检测试剂盒(JC-1)检测MMP的变化,采用荧光素酶法检测三磷酸腺苷(ATP)含量.结果 相较于NC组小鼠,HFD组小鼠的体重、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、血清中总胆固醇(TC)含量以及肝脏内的脂质沉积均明显增加(P<0.05),HFD组小鼠下丘脑SOD酶活性降低(P<0.05),而MDA含量升高(P<0.05),HFD组小鼠下丘脑线粒体水肿、线粒体嵴断裂,线粒体膜电位及ATP含量下降(P<0.05).结论 高脂饮食诱导的肥胖引起小鼠下丘脑线粒体结构紊乱及功能障碍.
Abstract
Objective To study the effect of obesity induced by high fat diet on the structure and function of hypothalamus mi-tochondria in mice.Methods Twelve male C57BL/6J mice,aged six weeks,were randomly assigned to two groups:a group fed with a high-fat diet(HFD)and a group fed with a normal diet(NC).After 12 weeks of being on their respective diets,various measurements such as body weight,blood lipid levels,Hematoxylin-Eosin staining(HE),and Oil-red O staining of the liver were conducted in order to assess the successful establishment of an obesity model.The presence of ROS was identified using a reactive oxygen species(ROS)detection kit,while the levels of MDA and SOD were measured using malondialdehyde(MDA)and superoxide dismutase(SOD)kits respectively.The transmission electron microscope was utilized to observe the ultrastructure of mitochondria in the hypothalamus,and the mitochondrial membrane potential(MMP)was assessed using an MMP(JC-1)detection kit.Additionally,luciferase enzyme was em-ployed to measure ATP content.Results Compared with NC group,body weight,serum total cholesterol(TC),high density lipoprotein(HDL),low density lipoprotein(LDL)contents and liver lipid deposition of HFD group were significantly increased(P<0.05),hypo-thalamic SOD activity of HFD group was decreased(P<0.05),while MDA content was increased(P<0.05).HFD group mice hypo-thalamus mitochondrial edema,mitochondrial crest fracture,mitochondrial membrane potential and ATP content decreased(P<0.05).Conclusion Obesity induced by high-fat diet can cause structural disorder and dysfunction of hypothalamic mitochondria in mice.
关键词
高脂饮食/肥胖/下丘脑/线粒体Key words
High-fat diet/Obesity/Hypothalamus/Mitochondria引用本文复制引用
出版年
2024
NETL
NSTL
万方数据