HSP90在糖尿病大鼠创面修复过程中的作用
Effects of HSP90 in the process of wound repair in diabetic rats
张汉霖 1曹彩霞 2高剑 1李晓亮 1吴建科 1马国续 1黄永禄 1巩凡1
作者信息
- 1. 宁夏回族自治区人民医院手足和重建显微外科,宁夏银川 750002
- 2. 宁夏回族自治区人民医院健康管理中心,宁夏 银川 750002
- 折叠
摘要
目的 研究HSP90 在糖尿病大鼠创面修复过程中的作用.方法 选取健康成年雄性SD大鼠,向其腹腔注射链脲佐菌素(STZ),背部打孔(直径2 cm),形成创面后,将大鼠随机分为对照组、空白凡士林治疗组(凡士林组)、胰岛素凡士林治疗组(胰岛素组)、HSP90 凡士林治疗组(HSP90 组),分别进行治疗,第3、5、10、15 d在同一深度、同一方向切取背部创面.通过对模型大鼠创面的愈合率观察、创面组织的苏木精-伊红(HE)染色及免疫组化染色与Western bloting法检测创面组织中HSP90 的表达.结果 HSP90 治疗可以增加糖尿病大鼠创面组织愈合率,与对照组及凡士林组相比,差异具有统计学意义(P<0.05).随着治疗时间的延长,HSP90 治疗后创面愈合效果较胰岛素治疗创面愈合更显著(P<0.05).结论 HSP90 局部创面使用可促进糖尿病大鼠创面愈合,其作用可能与创面组织HSP90 表达增高有关.
Abstract
Objective To investigate the role of HSP 90 in the process of wound repair in diabetic rats.Methods Healthy adult male SD rats were selected and treated with intraperitoneal injection of streptozotocin(STZ),dorsal perforation(2cm in diameter),and formation of trauma,and then the rats were randomly divided into a control group,a blank vaseline treatment group(vaseline group),an insulin vaseline treatment group(insulin group),and an HSP90 vaseline treatment group(HSP90 group)at the same depth and in the same direction on the 3 rd,5 th,10 th,and 15 th day of treatment,respectively.The healing rate of the wounds,HE staining and im-munohistochemical staining of the wounds,and western bloting were used to detect the expression of HSP90 protein in the wounds of the model rats.Results Comparing with the control and vaseline groups,the HSP90 treatment increased the rate of wound tissue healing in diabetic rats,and the difference was statistically significant(P<0.05).With the increase of treatment time,the wound healing after HSP90 treatment was more significant than that of insulin treatment(P<0.05).Conclusion The topical wound application of HSP90 may promote wound healing in diabetic rats,and its effect may be related to the increased expression of HSP90 in the wound tissues.
关键词
糖尿病大鼠/糖尿病创面/创面修复/HSP90Key words
Diabetes rats/Diabetes wound/Wound repair/HSP90引用本文复制引用
基金项目
宁夏自然科学基金项目(2022AAC03359)
宁夏自然科学基金项目(2023AAC03493)
宁夏回族自治区人民医院培育振兴项目(202010)
出版年
2024
NETL
NSTL
万方数据