Effects of HSP90 in the process of wound repair in diabetic rats
Objective To investigate the role of HSP 90 in the process of wound repair in diabetic rats.Methods Healthy adult male SD rats were selected and treated with intraperitoneal injection of streptozotocin(STZ),dorsal perforation(2cm in diameter),and formation of trauma,and then the rats were randomly divided into a control group,a blank vaseline treatment group(vaseline group),an insulin vaseline treatment group(insulin group),and an HSP90 vaseline treatment group(HSP90 group)at the same depth and in the same direction on the 3 rd,5 th,10 th,and 15 th day of treatment,respectively.The healing rate of the wounds,HE staining and im-munohistochemical staining of the wounds,and western bloting were used to detect the expression of HSP90 protein in the wounds of the model rats.Results Comparing with the control and vaseline groups,the HSP90 treatment increased the rate of wound tissue healing in diabetic rats,and the difference was statistically significant(P<0.05).With the increase of treatment time,the wound healing after HSP90 treatment was more significant than that of insulin treatment(P<0.05).Conclusion The topical wound application of HSP90 may promote wound healing in diabetic rats,and its effect may be related to the increased expression of HSP90 in the wound tissues.
NETL
NSTL
万方数据
