Effect of Lycium barbarum polysaccharides on retinal ganglion cells after optic nerve injury in rats
Objective To observe the protective effect of lycium barbarum polysaccharides(LBP)on retinal ganglion cells(RGCs)after optic nerve injury in adult rats.Methods 48 healthy male SD rats were randomly divided into four groups:normal group(A),injury group(B),LBP treatment group(C)and LBP prevention group(D),with 12 rats in each group.Except for group A,the other groups were used mini-clamp to make the injured optic nerve rat models on the left eye.Group D was given LBP 100 mg/(kg·d-1)for 2 weeks before modeling,and for 3 months after modeling.Group B and C were respectively given the normal sa-line and LBP 100 mg/(kg·d-1)for 3 months after modeling.Fluorescent gold(FG)was injected into the superior colliculi bilaterally to label RGCs one week before execution.The left eyeball of rats was removed after intragastric administration for three months.The retina was stripped and spread for calculating the density of RGCs.Meanwhile,the contents of superoxide dismutase(SOD)and malondialde-hyde(MDA)in the retina were detected.Results The densities of RGC in group A,B,C and group D were(2 354±250),(802±82),(1 138±96)and(1 196±82)cells/mm2 respectively.There was a statistically significant difference among the four groups(P＜0.05).The density of RGCs in group B decreased,with different sizes and shapes,the staining of RGC was weak and their arrangement was irregular.The contents of SOD in group A,B,C and group D were(77.93±9.64),(24.23±7.44),(40.47±16.94)and(42.15±8.87)U/mgprot respectively.There was a statistically significant difference among the four groups(P＜0.05).The contents of MDA in group A,B,C and group D were(6.21±1.97),(43.09±13.15),(29.55±4.51)and(28.91±6.74)nmol/mgprot respectively.There was a statistically significant difference among the four groups(P＜0.05).Conclusion LBP enhances the ability to scavenge reactive oxygen species(ROS)by up-regulating the content of SOD in RGCs after TON,inhibits lipid peroxidation reaction and down-regulates the content of MDA.It significantly increases the density of RGCs labeled by FG and has a protective effect on the apoptosis of RGCs induced by TON.
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