Effects of silencing CREPT on the proliferation and apoptosis and β-catenin expression of renal cell carcinoma
Objective To investigate the expression of CREPT in renal cell carcinoma and adjacent tissues and its correlation with common clinicopathological factors,to further study the effect of silencing CREPT on the proliferation and apoptosis of renal cell carci-noma cells,and to explore its relationship with β-catenin relation.Methods 75 pairs of renal clear cell carcinoma and paracancerous tis-sue microarrays undergoing radical resection were collected and were examined by using immunohistochemistry technology,and its expres-sion in renal cell carcinoma and paracancerous tissues were examined,and CREPT and common clinicopathological factors in renal cell car-cinoma relevance was study.At the same time,gene silencing technology was used to study the effect of CREPT gene on the proliferation and apoptosis of renal cell carcinoma cells,and the effect of CREPT silencing on the expression of β-catenin in 786-O cells was explored.Results The results showed that CREPT was highly expressed in renal cell carcinoma and lower in adjacent tissues,and was different in pathological grading and clinical grading and staging(P＜0.05);After silencing the CREPT gene,it was found that the proliferation of renal cell carcinoma was inhibited and promoted(P＜0.05).After silencing the CREPT gene,it was found that the expression of p-β-catenin was down-regulated(P＜0.05).Conclusion Silencing CREPT could inhibit the proliferation of renal cancer cells and promotes the apoptosis of renal cell cancer cells;CREPT may be involved in Wnt/β-catenin signaling pathway promotes renal tumorigenesis.
万方数据
维普
