[Aims] This study aims to screen out homemade seed coating formulation with high efficacy against Rhizoctonia solani to promote seed germination and seedling growth.[Methods] The laboratory plate growth rate methodin vitro,indoor seed coating method and field plot test were adopted.[Results] Among 20 kinds of homemade seed coating formulations,M1,M11,M13,M14,M17 had better effect to R.solani,their EC5o values were 79.4,130.2,470.2,350.4,152.9 mg/L,respectively.The cotton seed coated with M1 indicated the highest seed vigor,the germination potential and germination rate,root shoot ratio,all increased by 5.08,3.62,28.89%;compared to CK1 (commercially available) and increased by 24.63,26.13,68.59% compared with CK2 (without coating),and the control effect amounted to 72.75%,higher than CK1 (commercially available) by 6.58%;followed by M11,germination potential,germination rate,shoot CK1 (commercially available) had no significant difference,compared with CK2 (without coating).[Conclusions] M1 seed coating formulation has the best inhibitory effect on R.solani,no inhibition on seed germination and seedling growth,good safety and high control efficacy,followed by M11 seed coating formulation.