[目的]评价阿维菌素在烟草上使用的安全性,建立了超高效液相色谱-串联质谱(UPLC-MS/MS)检测烟草中阿维菌素的农药残留分析方法,并开展阿维菌素在烟草中的残留量与残留降解研究.[方法]消解试验按照阿维菌素30、45 g a.i./hm2 一次施药于烟株,定期取样;最终残留试验按照阿维菌素30(1.0倍推荐高剂量)、45 g a.i./hm2(1.5倍推荐高剂量)喷雾施药1次,施药后第28天取样.超高效液相色谱-串联质谱法对阿维菌素进行定量分析.[结果]田间试验消解动态结果表明:阿维菌素在烟叶中消解较快,消解半衰期为2.98～3.1 d,施药后28 d,烟叶中消解率达99.64％～99.80％.按照推荐剂量高剂量的1.0倍和1.5倍于烟草旺长期施药,距离末次施药的第28天采样,烟叶中阿维菌素的残留量远低于蔬菜水果残留限量标准.[结论]所建立方法的精密度、准确度、灵敏度均符合农药残留分析检测要求;阿维菌素在烟草中消解速率较快,属于易降解农药,推荐其在烟草上施用的使用剂量为30 g a.i./hm2,施药次数为1次,安全间隔期为28 d,可用于防治烟草病虫害发生.
Residue analysis and dissipation dynamics of abamectin in tobacco
[Aims]In order to evaluate the safety of abamectin in tobacco,an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was established to detect the pesticide residues of abamectin in tobacco,and the residues and degradation of abamectin in tobacco were studied.[Methods]In the dissipation experiment,abamectin was applied once at 30 and 45 g a.i./ha to the tobacco plants,and the samples were taken at regular intervals;the final residue experiment was conducted by applying abamectin at 30 g a.i./ha(recommended high dose)and 45 g a.i./ha(1.5 times the recommended high dose)by spraying once,and the samples were taken on the 28th day after application.The abamectin was quantified by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).[Results]The results of the dissipation dynamics of the field test showed that abamectin was rapidly eliminated in the tobacco leaves,with the half-life of elimination ranging from 2.98 to 3.1 d.On day 28 after application,the dissipation rate in tobacco leaves was 99.64％-99.80％.Applying abamectin at 1.0 and 1.5 times recommended high dosage in the tobacco peak period,on the 28th day after the last application,the residual amount of abamectin in the tobacco leaves was far lower than the residue limit standard for vegetables and fruits.[Conclusions]The precision,accuracy and sensitivity of the established method meet the requirements of pesticide residue analysis and detection;abamectin has a fast dissipation rate in tobacco and belongs to easily degradable pesticides.It is recommended to apply it on tobacco at 30 g a.i./ha,with a frequency of one and a preharvest interval of 28 d,to control the occurrence of tobacco diseases and insect pests.
abamectintobaccoultra performance liquid chromatography-tandem mass spectrometrydissipation dynamicsfinal residue
NETL
NSTL
万方数据
维普
