Effect of bacterial complex YM4 on degradation of nicosulfuron and soil microorganisms
[Aims]This study aims to construct nicosulfuron-degrading composite bacteria to promote the growth of maize and remediate contaminated soil.[Methods]Bacillus cereus N1 and Serratia marcescens N80 were mixed,the combinition ratio,medium optimization,degradation characteristics,growth promotion ability and soil remediation colonization were studied.[Results]B.cereus N1 and S.marcescens N80 were mixed according to 1:1 and named YM4.The optimum culture conditions were:20.00 g/L of soluble starch,10.00 g/L of potassium nitrate,and 5.50 g/L of calcium chloride,which degraded 92.96％of nicosulfuron.The optimum degradation conditions were:temperature of 35 ℃,pH 6.0,and substrate concentration of 50 mg/L.The growth-promoting ability of the composite bacteria was higher than that of the two single strains.The rifampicin-induced YM4L could colonize well in the inter-root and soil of maize,which could improve the soil microbial population and repair the soil.[Conclusions]The composite bacteria YM4 improved the degradation and bioconcentration ability compared with the N1 or N80,which provides a theoretical basis for the improvement of bioremediation of nicosulfuron-contaminated soils.
万方数据
维普
