首页|38％唑醚·啶酰菌WG应用后烟叶叶际微生物的变化规律

38％唑醚·啶酰菌WG应用后烟叶叶际微生物的变化规律

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[目的]为评价复配剂38％唑醚·啶酰菌WG防控烟草靶斑病的应用前景.[方法]采用菌丝生长速率法测定了其原药吡唑醚菌酯、啶酰菌胺及其制剂38％唑醚·啶酰菌WG对烟草靶斑病菌的抑菌活性;并利用Illumina Hiseq高通量测序技术分析了 38％唑醚·啶酰菌WG应用后,烟叶叶际真菌和细菌群落结构和多样性的变化规律.[结果]吡唑醚菌酯、啶酰菌胺和38％唑醚·啶酰菌WG对烟草靶斑病菌菌丝生长均具有较强的抑菌活性,其EC50值分别为0.26、1.58、0.24mg/L.施药前,靶斑病烟叶叶际优势真菌属为亡革菌属、葡萄孢属和尾孢属,优势细菌属为假单胞菌属、甲基杆菌属、泛菌属和鞘氨醇单胞菌属.施药后,烟叶真菌和细菌群落结构与多样性均存在变化.其中,施药后感病组织亡革菌属相对丰度显著降低;药后1、3d时马赛菌属等3种细菌相对丰度显著降低,9、18d时泛菌属等6种细菌的相对丰度均降低;镰刀菌属等7种真菌菌属施药后相对丰度先升高后降低,甲基杆菌属等4种细菌的相对丰度增加.[结论]揭示了 38％唑醚·啶酰菌WG施用后不同时期感病与健康烟叶组织叶际微生态变化差异,为该药剂防控烟草靶斑病的应用提供了参考依据.
Changes of phyllosphere microorganisms in tobacco leaves after the application of pyraclostrobin·boscalid 38％WG
[Aims]This study aims to evaluate the application prospect of pyraclostrobin·boscalid 38％WG for the control of tobacco target spot.[Methods]The mycelial growth rate method was used to determine the inhibitory activities of pyraclostrobin,boscalid and pyraclostrobin boscalid 38％WG against tobacco target spot pathogens;and the changing rules of the structure and diversity of the phyllosphere fungal and bacterial communities in tobacco were analyzed after the application of pyraclostrobin·boscalid 38％WG by the Illumina Hiseq high-throughput sequencing technology.[Results]Pyraclostrobin,boscalid and pyraclostrobin·boscalid 38％WG all had strong activities against the mycelium growth of tobacco target spot pathogen.The EC50 values were 0.26,1.58 and 0.24 mg/L.Before application of fungicides,the predominant phyllotropic fungi of tobacco leaves at the damage stage of tobacco target spot were Thanatephorus,Botrytis and Cercospora,and the dominant bacterial genera were Pseudomonas,Methylbacterium,Pantoea and Sphingomonas.The structure and diversity of fungal and bacterial communities in infected and healthy tobacco leaves were altered upon application.Among them,the relative abundance of Thanatephorus was significantly reduced in susceptible areas after application;the relative abundance of 3 species of bacteria including Masseillae.was significantly reduced on day 1 and 3 after application,and that of 6 species of bacteria including Pantoea.reduced on day 9 and 18 after application;the relative abundance of 7 fungal genera including Fusarium.increased and then decreased,and that of 4 species of bacteria including Methylobacterium.increased after application.[Conclusions]The differences in the phyllosphere microecological changes between diseased and healthy tobacco tissues at different periods after the application of pyraclostrobin·boscalid 38％WG were revealed,which provides a reference basis for the application of this agent for the prevention and control of tobacco target spot disease.

tobacco target spot diseasepyraclostrobinboscalidphyllospherehigh-throughput sequencing

张兴红、汪汉成、张艺、郭沫言、邱雪柏、王丰

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贵州大学农学院,贵阳 550025

贵州省烟草科学研究院,贵阳 550081

长江大学农学院,湖北荆州 434025

烟草靶斑病 吡唑醚菌酯 啶酰菌胺 叶际微生态 高通量测序

国家自然科学基金国家自然科学基金贵州省科技基金中国烟草总公司科技项目中国烟草总公司科技项目中国烟草总公司科技项目贵州省"百层次"创新型人才中国烟草总公司贵州省公司项目

3196055032160522黔科合基础-ZK[2021]重点036110202001035LS-04110202101048LS-08110202101045LS-05黔科合平台人才-GCC[2022]028-12021XM16

2024

10.16820/j.nyzz.2024.0111

农药
沈阳化工研究院有限公司

农药

CSTPCD北大核心
影响因子：0.665
ISSN：1006-0413
年,卷(期)：2024.63(1)
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