Temperature coefficient in bioactivity of tetrachlorantraniliprole and chlorfenapyr to Plutella xylostella by different bioassay methods
[Aims]To evaluate the influence of different bioassay methods on the insecticide temperature effect.[Methods]The toxicity of tetrachlorantraniliprole and chlorfenapyr against Plutella xylostella were determined by leaf-dipping method,insect-dipping method and topical application method,respectively,and the temperature-dependent trends were analyzed.[Results]For positive temperature coefficient insecticide tetrachlorantraniliprole,a 99.15-fold increase in toxicity with increasing temperature(15-35 ℃)was observed with the leaf-dipping method,showing a strong positive temperature effect,while the other two methods showed a weak positive temperature effect.Chlorfenapyr,whose biological activity was not affected by temperature in leaf-dipping assay,showed a weak positive temperature effect of+5.50 and+3.50 when using the insect-dipping and topical application method.When use leaf-dipping method,the temperature coefficients of tetrachlorantraniliprole and chlorfenapyr were+99.15 and+1.58 at 35 ℃,respectively,with significant differences.In contrast,the temperature coefficients of the two insecticides against P.xylostella were similar when using the insect-dipping and topical application methods.[Conclusions]The choice of bioassay method significantly affected the temperature coefficient of tetrachlorantraniliprole,which had a positive temperature effect to diamothback moth,whereas temperature independent pesticide,chlorfenapyr,was not significantly affected on the bioactivity.
万方数据
维普
