HPLC法分析0.6％苦参碱·丁子香酚可溶液剂
Analysis of matrine·eugenol 0.6％SL by HPLC
包娜 1邓颖 1何钰 1许筱莉 1杨昌彪 1马凯 1张振1
作者信息
- 1. 贵州省分析测试研究院,贵阳 550002
- 折叠
摘要
[目的]建立一种同时分析0.6％苦参碱·丁子香酚可溶液剂中有效成分含量的高效液相色谱法.[方法]以甲醇-0.4％磷酸氢二钠水溶液为流动相,梯度洗脱,采用C18色谱柱和DAD检测器在215 nm处对试样中的槐定碱、苦参碱和丁子香酚进行同时分离测定,外标法定量.[结果]采用该分析方法,槐定碱、苦参碱、丁子香酚能得到很好的分离,在所选的质量浓度范围内线性关系良好,槐定碱、苦参碱、丁子香酚的决定系数分别为0.9998、0.9998、0.9997,相对标准偏差分别为0.38％、1.00％、0.61％,平均回收率分别为99.6％、99.9％、99.9％.[结论]该方法操作简便、精密度和准确度高、分离效果好,可用于0.6％苦参碱·丁子香酚可溶液剂产品的质量控制.
Abstract
[Aims]This study aims to establish a method for the simultaneous determination of active components in matrine·eugenol 0.6％SL by HPLC.[Methods]Sophoridine,matrine and eugenol in the samples were simultaneously separated and determined by gradient elution,with the mixture of methanol and 0.4％disodium hydrogen phosphate aqueous solution as mobile phase,with C18 column and DAD detector at 215 nm,and quantified by external standard method.[Results]Sophoridine,matrine,and eugenol were well separated by using this method,the linear relationship were good within the selected mass concentration range,the determination coefficients of sophoridine,matrine and eugenol were 0.9998,0.9998 and 0.9997,the relative standard deviation were 0.38％,1.00％and 0.61％,and the average recoveries were 99.6％,99.9％and 99.9％,respectively.[Conclusions]The method is simple and convenient,with good precision,accuracy and separation effect.It can be used for quality control of matrine·eugenol 0.6％SL.
关键词
高效液相色谱/槐定碱/苦参碱/丁子香酚/同时测定Key words
HPLC/sophoridine/matrine/eugenol/simultaneous determination引用本文复制引用
基金项目
贵州省高层次创新型人才培养项目(千层次)()
贵州省科技计划项目(黔科院R字[2022]01号)
贵阳科技计划项目(筑科合同[2023]6-3)
出版年
2024
NETL
NSTL
维普
万方数据