10种杀菌剂对雪茄烟软腐病室内生物活性测定及田间防效评价
Bioactivity in laboratory and field efficacy of 10 bactericides against cigar soft rot
卢超 1唐旭兵 2裴卫华 3魏环宇 1方保 2黄飞燕 1余磊 1王锦丹 1施竹凤 3任龙辉 2沈俊儒 2孔垂思3
作者信息
- 1. 昆明学院云南省都市特色农业工程技术研究中心,昆明 650214
- 2. 云南香料烟有限责任公司,云南保山 678000
- 3. 云南省农业科学院农业环境资源研究所,昆明 650205
- 折叠
摘要
[目的]进行了室内生物活性和田间药效试验,旨在测定10种常用杀菌剂对雪茄烟软腐病的效果,并筛选出在软腐病菌防控中具有高效、安全和低毒性的药剂.[方法]采用离体叶片刺伤接种法进行不同药剂对软腐病巴西果胶杆菌的生物活性测定,以及田间病害防治试验,筛选出对雪茄烟软腐病具有很好防控作用的药剂.[结果]室内生物活性测定结果表明:10种供试杀菌剂中20％春雷霉素WG和80％乙蒜素EC的抑菌效果最强,EC50值分别为0.4942、1.673 1mg/L;3％中生菌素WP和20％二氯异氰尿酸钠WP抑菌效果次之,EC50值分别为1.8406、2.4917mg/L.离体叶片和盆栽防效测定表明防效作用依次为20％春雷霉素WG＞3％中生菌素WP＞80％乙蒜素EC＞20％二氯异氰尿酸钠WP.田间药效试验结果表明第3次喷药后7d,20％春雷霉素WG防效达到59.61％.[结论]20％春雷霉素WG对巴西果胶杆菌抑菌效果较好,可用于防治雪茄烟软腐病.
Abstract
[Aims]Biological activity in laboratory and field efficacy trials were conducted to determine the efficacy of 10 commonly used bactericides against soft rot of cigar tobacco and to screen for agents with high efficiency,safety and low toxicity.[Methods]The bioactivity of different agents against Pectobacterium brasiliense was carried out by prick inoculation of isolated leaves,and field disease control tests were conducted to screen out agents with good prevention and control of soft rot of cigar tobacco.[Results]The results of bioassay showed that among the 10 tested bactericides,kasugamycin 20％WG and ethylicin 80％EC had the strongest inhibitory effect,with EC50 values of 0.4942 and 1.6731 mg/L,respectively;followed by zhongshengmycin 3％WP and sodium dichloroisocyanurate 20％WP,with EC50 values of 1.8406 and 2.4917 mg/L,respectively.Determination of control effects on isolated leaf and pot experiments showed that the order of control effects were kasugamycin 20％WG＞zhongshengmycin 3％WP＞ethylicin 80％EC＞sodium dichloroisocyanurate 20％WP.The results of the field efficacy test showed that on day 7 after spraying 3 times,the preventive effect of kasugamycin 20％WG reached 59.61％.[Conclusions]Kasugamycin 20％WG has a good antibacterial effect on P.brasiliense and can be used to control soft rot of cigar tobacco.
关键词
巴西果胶杆菌/雪茄烟软腐病/防效/杀菌剂Key words
Pectobacterium brasiliense/cigar tobacco soft rot/control efficacy/bactericide引用本文复制引用
基金项目
中国烟草总公司云南省公司科技重大项目(2022530000241005)
出版年
2024
NETL
NSTL
维普
万方数据