Bioactivity in laboratory and field efficacy of 10 bactericides against cigar soft rot
[Aims]Biological activity in laboratory and field efficacy trials were conducted to determine the efficacy of 10 commonly used bactericides against soft rot of cigar tobacco and to screen for agents with high efficiency,safety and low toxicity.[Methods]The bioactivity of different agents against Pectobacterium brasiliense was carried out by prick inoculation of isolated leaves,and field disease control tests were conducted to screen out agents with good prevention and control of soft rot of cigar tobacco.[Results]The results of bioassay showed that among the 10 tested bactericides,kasugamycin 20%WG and ethylicin 80%EC had the strongest inhibitory effect,with EC50 values of 0.4942 and 1.6731 mg/L,respectively;followed by zhongshengmycin 3%WP and sodium dichloroisocyanurate 20%WP,with EC50 values of 1.8406 and 2.4917 mg/L,respectively.Determination of control effects on isolated leaf and pot experiments showed that the order of control effects were kasugamycin 20%WG>zhongshengmycin 3%WP>ethylicin 80%EC>sodium dichloroisocyanurate 20%WP.The results of the field efficacy test showed that on day 7 after spraying 3 times,the preventive effect of kasugamycin 20%WG reached 59.61%.[Conclusions]Kasugamycin 20%WG has a good antibacterial effect on P.brasiliense and can be used to control soft rot of cigar tobacco.