摘要
[目的]水稻纹枯病是水稻主要病害之一,严重影响水稻的产量.宁南霉素(ningnanmycin)可有效防治多种农作物病害.[方法]测定了在感染纹枯病的水稻上喷施宁南霉素的生理生化指标;通过qRT-PCR分析了病程相关蛋白相关基因 PRl-α、PR-3和PR-5的表达.[结果]与 Rhizoctonia solani组相比,ningnanmycin+Rhizoctonia solani组水稻叶片的叶绿素a、叶绿素b、类胡萝卜素含量和β-l,3-葡聚糖酶、内切几丁质酶、PPO的活性在4～7d显著增加;MDA、O2-和H2O2的含量在3～7 d显著降低;PAL、SOD、POD和CAT的活性在3～7 d显著增加;ningnanmycin+Rhizoctonia solani组水稻叶片的OsPR1-α、OsPR-3和OsPR-5基因的相对表达量在3～7 d显著高于Rhizoctonia solani组.[结论]宁南霉素通过诱导产生病程相关蛋白和保护酶,增强植株寄主抗性,可有效控制立枯丝核菌引起的水稻纹枯病.
Abstract
[Aims]Rice(Oryza sativa L.)sheath blight is one of the major diseases of rice,seriously harming rice yield.Ningnanmycin can effectively prevent and control a variety of crop diseases.[Methods]This study measured the physiological and biochemical indicators on rice infected with Rhizoctonia solani after spraying ningnanmycin;the expression of disease course-related protein-related genes PR1-α,PR-3 and PR-5 was analyzed by qRT-PCR.[Results]Compared with the Rhizoctonia solani group,the contents of chlorophyll a,chlorophyll b,total carotenoid and the activities of β-1,3-glucanase,endochitinase and PPO in rice leaves of the ningnanmycin+R.solani group were significantly higher on day 4 to 7;the content of MDA,O2-,and H2O2 significantly decreased from day 3 to day 7;the activities of PAL,SOD,POD,and CAT significantly increased from day 3 to day 7;the relative expression of OsPR1-α,OsPR-3 and OsPR-5 in rice leaves of the ningnanmycin+R.solani group were significantly higher than those of the R.solani group from day 3 to day 7.[Conclusions]Ningnanmycin can effectively control rice sheath blight caused by R.solani by inducing the production of disease-related proteins and protective enzymes to enhance plant host resistance.
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