Determination of 10 HPPD inhibitors and a metabolite in cereals by HPLC-MS/MS
[Aims]A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established for simultaneous determination of 10 4-hydroxyphenylpyruvate dioxygenase(HPPD)inhibitor herbicides and a metabolite in cereals(wheat,corn and brown rice).[Methods]The samples were soaked by 3％formic acid aqueous solution(volume fraction),extracted by acetonitrile,purified by primary secondary amine(PSA)and octadecylsilane(C18),and detected by HPLC-MS/MS.[Results]Good linear relationship appeared in the mass concentration range of 1.5-100 pg/L for 10 HPPD inhibitor herbicides and the metabolite.The limits of quantitation(LOQs)of them were 0.01 mg/kg.At three spiked levels of 0.01,0.03 and 0.1 mg/kg,the average recoveries were 74.3％-112.7％in 3 cereal matrices,with relative standard deviations(RSDs)less than 8.7％.[Conclusions]The method is simple and effective,with high accuracy and good precision,which is suitable for determination of the ten HPPD inhibitor herbicides and the metabolite in cereals.
high performance liquid chromatography-tandem mass spectrometry4-hydroxyphenylpyruvate dioxygenase inhibitorherbicidecerealresidue
万方数据
维普
