Phytotoxicity of clopyralid in pepper and relieving effect of sodium nitrophenolate
[Aims]The aim of this study is to explore the phytotoxicity of clopyralid drift on adjacent pepper and the alleviating effect of sodium nitrophenolate on it.[Methods]Pepper Qingyun No.2 was used for the tests,and the drift of the liquid was simulated by spraying clopyralid diluent,and the changes of leaf and stem tissues before and after spraying sodium nitrophenolate were observed.The activity of antioxidant enzyme and contents of malondialdehyde,chlorophyll,carotenoid and glutathione in the leaves of pepper seedlings were measured and compared.[Results]Within 7 d after application,as the concentration of clopyralid increased,the phytotoxicity symptoms of pepper seedlings increased,the MDA content of the leaves gradually increased,the activity of CAT,POD,SOD and content of chlorophyll and carotenoid increased first and then decreased,and the content of GSH decreased with the increase of concentration within 3-7 d after application.When the concentration of clopyralid was 0.016 g/L,the new leaves of peppers malformed and yellowed,and the stem began to show damage.At a concentration of 0.800 g/L,7 d after application,the leaves were severely deformed and partially withered,the integrity of the stem was destroyed,and all the physiological indicators were significantly lower than those of the control groups.After spraying sodium nitrophenolate at a concentrations of 0.016 and 0.040 g/L,the phenomenon of phytotoxicity on pepper was alleviated obviously,with reducement of MDA content,and significantly increase of CAT,POD and SOD and content of chlorophyll,carotenoids and GSH.When the concentration of clopyralid was 0.800 g/L,Spraying of sodium nitrophenolate had no obvious relief effect.[Conclusions]The drift of clopyralid can cause phytotoxicity to adjacent peppers,and the higher the concentration the more serious the phytotoxicity.Spraying of sodium nitrophenolate can alleviate phytotoxicity symptoms of clopyralid to a certain extent.
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