Determination of(Z)-5-benzyl-1-methyl-N-nitro-1,3,5-triazinan-2-imine in clothianidin 0.5％granule by HPLC
[Aims]This study aims to establish a HPLC method for the determination of impurity(Z)-5-benzyl-1-methyl-N-nitro-1,3,5-triazinan-2-imide in clothianidin 0.5％granule.[Methods]Agela Durashell C18 column was used,acetonitrile-acetic acid 0.1％aqueous solution was used as mobile phase for gradient elution,and the flow rate was 1.0 mL/min.The method was verified by diode array detector(DAD)at wavelength of 275 nm for the inpurity.[Results]The linear correlation R2 was 0.9996,the standard deviation was 0.000 015,the coefficient of variation was 1.25％,and the average recovery was 99.88％,all of which met the requirements of quantitative analysis.[Conclusions]The effective separation of impurities from other components can be realized,and it is suitable for the quality detection of corresponding formulation.
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