Rapid detection of mixture of 377 pesticide residues in agricultural products by grouped isotope internal standard-ultra high performance liquid chromatography-tandem mass spectrometry
Rapid detection of mixture of 377 pesticide residues in agricultural products by grouped isotope internal standard-ultra high performance liquid chromatography-tandem mass spectrometry
[Aims]Based on ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),a rapid screening method through grouped isotope internal standard for the determination of 377 pesticides in agricultural products was established.[Methods]Ten samples composed a mixed sample.Negative leaf mustard and orange were used as the standard substrate for vegetables and fruits and the content of pesticide residues was quantified with grouped isotope internal standard.[Results]The limit of quantitation of the method was 0.5-5 μg/kg,the linear range of the method was 5-500 μg/L,and the correlation coefficient was greater than 0.990.The recovery was in the range of 73.9％-112.7％with the coefficient of variation of 1.1％-22.3％at the low spiked levels of 0.5-5 μg/kg.The recovery was in the range of 70.7％-114.5％with the coefficient of variation of 1.3％-17.6％at the medium spiked levels of 10 μg/kg.The recovery was in the range of 86.4％-107.2％with the coefficient of variation of 0.7％-11.0％at the high spiked level of 50 μg/kg,meeting the requirements of GB/T 27404-2008.[Conclusions]This method has high sensitivity,accuracy and stability.In practice,the detection efficiency is 2.5-10 times that of the conventional method,which has great application value,remarkable economic efficiency and social benefits.
关键词
分组同位素内标/混检快筛/超高效液相色谱-串联质谱/农产品/农药残留
Key words
grouped isotope internal standard/mixed detection and rapid screening/UPLC-MS/MS/agricultural product/pesticide residue
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